Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 711911..712536 Replicon chromosome
Accession NZ_CP089778
Organism Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)


Gene name vapC Uniprot ID G0TQA0
Locus tag LVJ73_RS03255 Protein ID WP_003403218.1
Coordinates 712135..712536 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ36
Locus tag LVJ73_RS03250 Protein ID WP_003403213.1
Coordinates 711911..712138 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ73_RS03225 (LVJ73_03220) 707090..707311 - 222 WP_003898525.1 DUF4926 domain-containing protein -
LVJ73_RS03230 (LVJ73_03225) 707453..708058 + 606 WP_003898526.1 hypothetical protein -
LVJ73_RS03235 (LVJ73_03230) 708077..710644 - 2568 WP_003900188.1 SEC-C metal-binding domain-containing protein -
LVJ73_RS03240 (LVJ73_03235) 710728..711477 + 750 WP_003898528.1 hypothetical protein -
LVJ73_RS03245 (LVJ73_03240) 711474..711716 + 243 WP_003403210.1 hypothetical protein -
LVJ73_RS03250 (LVJ73_03245) 711911..712138 + 228 WP_003403213.1 antitoxin VapB29 Antitoxin
LVJ73_RS03255 (LVJ73_03250) 712135..712536 + 402 WP_003403218.1 type II toxin-antitoxin system ribonuclease VapC29 Toxin
LVJ73_RS03260 (LVJ73_03255) 712665..712748 + 84 Protein_646 galactose-1-phosphate uridylyltransferase -
LVJ73_RS03265 (LVJ73_03260) 712767..713848 + 1082 Protein_647 galactose-1-phosphate uridylyltransferase -
LVJ73_RS03270 (LVJ73_03265) 713845..714936 + 1092 WP_003906403.1 galactokinase -
LVJ73_RS03275 (LVJ73_03270) 715331..716395 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ73_RS03280 (LVJ73_03275) 716499..717446 + 948 WP_003403232.1 DUF732 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-120)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 13937.76 Da        Isoelectric Point: 5.7441

>T229033 WP_003403218.1 NZ_CP089778:712135-712536 [Mycobacterium tuberculosis]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Download         Length: 402 bp

>T229033 NZ_CP124373:c139339-139034 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8163.17 Da        Isoelectric Point: 5.6307

>AT229033 WP_003403213.1 NZ_CP089778:711911-712138 [Mycobacterium tuberculosis]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Download         Length: 228 bp

>AT229033 NZ_CP124373:c139559-139341 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQA0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSC9

References