Detailed information of TA system TA229027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 443972..444983
• Replicon: chromosome
• Accession: NZ_CP089778
• Organism: Mycobacterium tuberculosis strain I0003758-5

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: L7N5R2
• Locus tag: LVJ73_RS01930
• Protein ID: WP_003401859.1
• Coordinates: 443972..444565 (-)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: LVJ73_RS01935
• Protein ID: WP_003401860.1
• Coordinates: 444594..444983 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ73_RS01910 (LVJ73_01910)	439621..441003	+	1383	WP_003898415.1	magnesium transporter	-
LVJ73_RS01915 (LVJ73_01915)	441015..442049	-	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
LVJ73_RS01920 (LVJ73_01920)	442145..442828	+	684	WP_003401857.1	VTT domain-containing protein	-
LVJ73_RS01925 (LVJ73_01925)	442817..443947	-	1131	WP_003401858.1	glycoside hydrolase family 76 protein	-
LVJ73_RS01930 (LVJ73_01930)	443972..444565	-	594	WP_003401859.1	zeta toxin family protein	Toxin
LVJ73_RS01935 (LVJ73_01935)	444594..444983	-	390	WP_003401860.1	hypothetical protein	Antitoxin
LVJ73_RS01940 (LVJ73_01940)	445064..446275	-	1212	WP_003906353.1	VWA domain-containing protein	-
LVJ73_RS01945 (LVJ73_01945)	446281..446883	-	603	WP_003898417.1	SRPBCC family protein	-
LVJ73_RS01950 (LVJ73_01950)	446897..447793	-	897	WP_003898418.1	MoxR family ATPase	-
LVJ73_RS01955 (LVJ73_01955)	447790..448383	-	594	WP_003401864.1	NTP transferase domain-containing protein	-
LVJ73_RS01960 (LVJ73_01960)	448380..449135	-	756	WP_003401865.1	XdhC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21344.53 Da
• Isoelectric Point: 10.5018

>T229027 WP_003401859.1 NZ_CP089778:c444565-443972 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDNSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T229027 NZ_CP124372:c4026822-4026358 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT229027 WP_003401860.1 NZ_CP089778:c444983-444594 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT229027 NZ_CP124372:c4027157-4026822 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U4BRU8

Antitoxin

• AlphaFold DB: A0A7U4BRT0