Detailed information of TA system TA229021

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-PHD
• Location: 3818143..3818810
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: O50411
• Locus tag: LVJ72_RS18010
• Protein ID: WP_003417916.1
• Coordinates: 3818143..3818535 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A0E8U8S7
• Locus tag: LVJ72_RS18015
• Protein ID: WP_003912214.1
• Coordinates: 3818535..3818810 (-)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS17990	3813515..3815125	-	1611	WP_003417910.1	1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein	-
LVJ72_RS17995	3815070..3815351	-	282	WP_003914149.1	1-deoxy-D-xylulose-5-phosphate synthase N-terminal domain-containing protein	-
LVJ72_RS18000 (LVJ72_18005)	3815348..3816337	-	990	WP_003417912.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ72_RS18005 (LVJ72_18010)	3816337..3817389	-	1053	WP_003900678.1	polyprenyl synthetase family protein	-
LVJ72_RS18010 (LVJ72_18015)	3818143..3818535	-	393	WP_003417916.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ72_RS18015 (LVJ72_18020)	3818535..3818810	-	276	WP_003912214.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ72_RS18020 (LVJ72_18025)	3818992..3820363	+	1372	Protein_3560	ISNCY family transposase	-
LVJ72_RS18025 (LVJ72_18030)	3820553..3822757	+	2205	WP_031664069.1	PE family protein	-
LVJ72_RS18030 (LVJ72_18035)	3822828..3823700	-	873	WP_003417929.1	3-hydroxyacyl-thioester dehydratase HtdY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3819686..3820363	677

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14316.74 Da
• Isoelectric Point: 10.7249

>T229021 WP_003417916.1 NZ_CP089777:c3818535-3818143 [Mycobacterium tuberculosis]
MAAIYLDSSAIVKLAVREPESDALRRYLRTRHPRVSSALARAEVMRALLDKGESARKAGRRALAHLDLLRVDKRVLDLAG
GLLPFELRTLDAIHLATAQRLGVDLGRLCTYDDRMRDAAKTLGMAVIAPS

Nucleotide

• Length: 393 bp

>T229021 NZ_CP124372:2811562-2811939 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 9966.35 Da
• Isoelectric Point: 4.5332

>AT229021 WP_003912214.1 NZ_CP089777:c3818810-3818535 [Mycobacterium tuberculosis]
MTSVGVRALRQRASELLRRVEAGETIEITDRGRPVALLSPLPQGGPYEQLLASGEIERATLDVVDLPEPLDLDAGVELPS
VTLARLREHER

Nucleotide

• Length: 276 bp

>AT229021 NZ_CP124372:2811138-2811515 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Toxin

• AlphaFold DB: A0A7U4BXS8

Antitoxin

• AlphaFold DB: A0A0E8U8S7