Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3200567..3201254 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag LVJ72_RS15285 Protein ID WP_003414624.1
Coordinates 3200811..3201254 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag LVJ72_RS15280 Protein ID WP_003414620.1
Coordinates 3200567..3200824 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS15260 (LVJ72_15260) 3195887..3196741 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ72_RS15265 (LVJ72_15265) 3196797..3197960 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ72_RS15270 (LVJ72_15270) 3197977..3199191 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ72_RS15275 (LVJ72_15275) 3199199..3200440 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
LVJ72_RS15280 (LVJ72_15280) 3200567..3200824 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
LVJ72_RS15285 (LVJ72_15285) 3200811..3201254 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ72_RS15290 (LVJ72_15290) 3201334..3201996 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
LVJ72_RS15295 (LVJ72_15295) 3202092..3202283 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
LVJ72_RS15300 (LVJ72_15300) 3202615..3204363 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
LVJ72_RS15305 (LVJ72_15305) 3204459..3205040 + 582 WP_003414644.1 fasciclin domain-containing protein -
LVJ72_RS15310 (LVJ72_15310) 3205140..3205406 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T229017 WP_003414624.1 NZ_CP089777:3200811-3201254 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T229017 NZ_CP124372:c2316760-2316476 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT229017 WP_003414620.1 NZ_CP089777:3200567-3200824 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT229017 NZ_CP124372:c2316996-2316763 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References