Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-Phd
Location 3194966..3195514 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name relE Uniprot ID G0TFG5
Locus tag LVJ72_RS15255 Protein ID WP_003414602.1
Coordinates 3195251..3195514 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID O33347
Locus tag LVJ72_RS15250 Protein ID WP_003414599.1
Coordinates 3194966..3195247 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS15225 (LVJ72_15225) 3190589..3191446 - 858 WP_003899513.1 type I methionyl aminopeptidase -
LVJ72_RS15230 (LVJ72_15230) 3191488..3192072 - 585 WP_003899514.1 DUF1707 domain-containing protein -
LVJ72_RS15235 (LVJ72_15235) 3192176..3192424 + 249 WP_003913411.1 antitoxin VapB23 -
LVJ72_RS15240 (LVJ72_15240) 3192421..3192801 + 381 WP_003414592.1 type II toxin-antitoxin system VapC family toxin -
LVJ72_RS15245 (LVJ72_15245) 3192883..3194694 - 1812 WP_003414596.1 penicillin-binding protein -
LVJ72_RS15250 (LVJ72_15250) 3194966..3195247 + 282 WP_003414599.1 type II toxin-antitoxin system antitoxin RelF Antitoxin
LVJ72_RS15255 (LVJ72_15255) 3195251..3195514 + 264 WP_003414602.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ72_RS15260 (LVJ72_15260) 3195887..3196741 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ72_RS15265 (LVJ72_15265) 3196797..3197960 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ72_RS15270 (LVJ72_15270) 3197977..3199191 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ72_RS15275 (LVJ72_15275) 3199199..3200440 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10204.82 Da        Isoelectric Point: 11.5078

>T229016 WP_003414602.1 NZ_CP089777:3195251-3195514 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Download         Length: 264 bp

>T229016 NZ_CP124372:c2049025-2048105 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10183.40 Da        Isoelectric Point: 4.3440

>AT229016 WP_003414599.1 NZ_CP089777:3194966-3195247 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Download         Length: 282 bp

>AT229016 NZ_CP124372:c2049794-2049012 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3G5O

Antitoxin

Source ID Structure
PDB 3G5O
AlphaFold DB A0A7U4BWP8

References