Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3154049..3154653 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag LVJ72_RS15055 Protein ID WP_003414492.1
Coordinates 3154049..3154441 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag LVJ72_RS15060 Protein ID WP_003414495.1
Coordinates 3154438..3154653 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS15025 (LVJ72_15030) 3149199..3149987 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
LVJ72_RS15030 (LVJ72_15035) 3150321..3150866 - 546 WP_003913758.1 DUF1802 family protein -
LVJ72_RS15035 (LVJ72_15040) 3151138..3152022 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LVJ72_RS15040 (LVJ72_15045) 3152025..3152912 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
LVJ72_RS15045 (LVJ72_15050) 3153217..3153762 - 546 WP_003899500.1 DUF1802 family protein -
LVJ72_RS15050 (LVJ72_15055) 3153759..3154028 - 270 WP_003414489.1 DUF2277 family protein -
LVJ72_RS15055 (LVJ72_15060) 3154049..3154441 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ72_RS15060 (LVJ72_15065) 3154438..3154653 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ72_RS15065 (LVJ72_15070) 3154700..3155449 + 750 WP_003414497.1 enoyl-CoA hydratase -
LVJ72_RS15070 (LVJ72_15075) 3155528..3156610 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
LVJ72_RS15075 (LVJ72_15080) 3156603..3157913 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
LVJ72_RS15080 (LVJ72_15085) 3157916..3158743 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
LVJ72_RS15085 (LVJ72_15090) 3158740..3159651 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T229015 WP_003414492.1 NZ_CP089777:c3154441-3154049 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T229015 NZ_CP124372:1865200-1865418 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT229015 WP_003414495.1 NZ_CP089777:c3154653-3154438 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT229015 NZ_CP124372:1864800-1865174 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References