Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 3086492..3087179 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID G0TF65
Locus tag LVJ72_RS14685 Protein ID WP_003414064.1
Coordinates 3086784..3087179 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TF64
Locus tag LVJ72_RS14680 Protein ID WP_003414061.1
Coordinates 3086492..3086758 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS14655 (LVJ72_14655) 3082131..3083033 - 903 WP_003900564.1 4-hydroxy-tetrahydrodipicolinate synthase -
LVJ72_RS14660 (LVJ72_14660) 3083102..3083854 - 753 WP_003899465.1 FAD-dependent thymidylate synthase -
LVJ72_RS14665 (LVJ72_14670) 3084098..3084373 - 276 WP_003414055.1 type I restriction endonuclease subunit S -
LVJ72_RS14670 (LVJ72_14675) 3084370..3085992 - 1623 WP_003906897.1 class I SAM-dependent DNA methyltransferase -
LVJ72_RS14675 (LVJ72_14680) 3086079..3086495 - 417 WP_003414059.1 type II toxin-antitoxin system toxin ribonuclease C21 -
LVJ72_RS14680 (LVJ72_14685) 3086492..3086758 - 267 WP_003414061.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ72_RS14685 (LVJ72_14690) 3086784..3087179 - 396 WP_003414064.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ72_RS14690 (LVJ72_14695) 3087176..3087445 - 270 WP_003414066.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ72_RS14695 (LVJ72_14700) 3087455..3088549 - 1095 WP_003414068.1 restriction endonuclease subunit S -
LVJ72_RS14700 (LVJ72_14705) 3088546..3088965 - 420 WP_003414070.1 winged helix-turn-helix domain-containing protein -
LVJ72_RS14705 (LVJ72_14710) 3088964..3089038 + 75 Protein_2902 hypothetical protein -
LVJ72_RS14710 (LVJ72_14715) 3089039..3089518 - 480 WP_003414073.1 dihydrofolate reductase -
LVJ72_RS14715 (LVJ72_14720) 3089589..3090389 - 801 WP_003911953.1 thymidylate synthase -
LVJ72_RS14720 (LVJ72_14725) 3090545..3091282 + 738 WP_003414079.1 dienelactone hydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(16-56)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14340.04 Da        Isoelectric Point: 4.7145

>T229012 WP_003414064.1 NZ_CP089777:c3087179-3086784 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Download         Length: 396 bp

>T229012 NZ_CP124372:676889-678211 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT229012 WP_003414061.1 NZ_CP089777:c3086758-3086492 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT229012 NZ_CP124372:676623-676889 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TF65

Antitoxin

Source ID Structure
AlphaFold DB G0TF64

References