Detailed information of TA system TA229011

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3086079..3086758
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: LVJ72_RS14675
• Protein ID: WP_003414059.1
• Coordinates: 3086079..3086495 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: LVJ72_RS14680
• Protein ID: WP_003414061.1
• Coordinates: 3086492..3086758 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS14655 (LVJ72_14655)	3082131..3083033	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
LVJ72_RS14660 (LVJ72_14660)	3083102..3083854	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
LVJ72_RS14665 (LVJ72_14670)	3084098..3084373	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
LVJ72_RS14670 (LVJ72_14675)	3084370..3085992	-	1623	WP_003906897.1	class I SAM-dependent DNA methyltransferase	-
LVJ72_RS14675 (LVJ72_14680)	3086079..3086495	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
LVJ72_RS14680 (LVJ72_14685)	3086492..3086758	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ72_RS14685 (LVJ72_14690)	3086784..3087179	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
LVJ72_RS14690 (LVJ72_14695)	3087176..3087445	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ72_RS14695 (LVJ72_14700)	3087455..3088549	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
LVJ72_RS14700 (LVJ72_14705)	3088546..3088965	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
LVJ72_RS14705 (LVJ72_14710)	3088964..3089038	+	75	Protein_2902	hypothetical protein	-
LVJ72_RS14710 (LVJ72_14715)	3089039..3089518	-	480	WP_003414073.1	dihydrofolate reductase	-
LVJ72_RS14715 (LVJ72_14720)	3089589..3090389	-	801	WP_003911953.1	thymidylate synthase	-
LVJ72_RS14720 (LVJ72_14725)	3090545..3091282	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T229011 WP_003414059.1 NZ_CP089777:c3086495-3086079 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T229011 NZ_CP124372:395066-395168 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT229011 WP_003414061.1 NZ_CP089777:c3086758-3086492 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT229011 NZ_CP124372:c395206-394938 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64