Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2886947..2887584 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag LVJ72_RS13590 Protein ID WP_003413180.1
Coordinates 2886947..2887342 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag LVJ72_RS13595 Protein ID WP_003413183.1
Coordinates 2887339..2887584 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS13550 (LVJ72_13550) 2882350..2883561 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ72_RS13555 (LVJ72_13555) 2883688..2884347 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ72_RS13560 (LVJ72_13560) 2884344..2885006 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ72_RS13565 (LVJ72_13565) 2885003..2885281 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ72_RS13570 (LVJ72_13570) 2885374..2885787 + 414 WP_003413164.1 PIN domain nuclease -
LVJ72_RS13575 (LVJ72_13575) 2885826..2886083 + 258 WP_003413167.1 CopG family transcriptional regulator -
LVJ72_RS13580 (LVJ72_13580) 2886080..2886457 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
LVJ72_RS13585 (LVJ72_13585) 2886473..2886847 - 375 WP_003413177.1 hypothetical protein -
LVJ72_RS13590 (LVJ72_13590) 2886947..2887342 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
LVJ72_RS13595 (LVJ72_13595) 2887339..2887584 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
LVJ72_RS13600 (LVJ72_13600) 2887995..2888414 - 420 WP_003413190.1 A24 family peptidase -
LVJ72_RS13605 (LVJ72_13605) 2888426..2889235 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ72_RS13610 (LVJ72_13610) 2889232..2890485 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ72_RS13615 (LVJ72_13615) 2890478..2890990 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T229008 WP_003413180.1 NZ_CP089777:c2887342-2886947 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T229008 NZ_CP124368:c87235-86960 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT229008 WP_003413183.1 NZ_CP089777:c2887584-2887339 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT229008 NZ_CP124368:c87519-87235 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References