Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2885826..2886457 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ72_RS13580 Protein ID WP_003413174.1
Coordinates 2886080..2886457 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ72_RS13575 Protein ID WP_003413167.1
Coordinates 2885826..2886083 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS13545 (LVJ72_13545) 2881740..2882054 + 315 WP_009937839.1 hypothetical protein -
LVJ72_RS13550 (LVJ72_13550) 2882350..2883561 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ72_RS13555 (LVJ72_13555) 2883688..2884347 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ72_RS13560 (LVJ72_13560) 2884344..2885006 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ72_RS13565 (LVJ72_13565) 2885003..2885281 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ72_RS13570 (LVJ72_13570) 2885374..2885787 + 414 WP_003413164.1 PIN domain nuclease -
LVJ72_RS13575 (LVJ72_13575) 2885826..2886083 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ72_RS13580 (LVJ72_13580) 2886080..2886457 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ72_RS13585 (LVJ72_13585) 2886473..2886847 - 375 WP_003413177.1 hypothetical protein -
LVJ72_RS13590 (LVJ72_13590) 2886947..2887342 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ72_RS13595 (LVJ72_13595) 2887339..2887584 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ72_RS13600 (LVJ72_13600) 2887995..2888414 - 420 WP_003413190.1 A24 family peptidase -
LVJ72_RS13605 (LVJ72_13605) 2888426..2889235 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ72_RS13610 (LVJ72_13610) 2889232..2890485 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ72_RS13615 (LVJ72_13615) 2890478..2890990 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T229007 WP_003413174.1 NZ_CP089777:2886080-2886457 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T229007 NZ_CP124368:80093-80509 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT229007 WP_003413167.1 NZ_CP089777:2885826-2886083 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT229007 NZ_CP124368:79866-80096 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References