Detailed information of TA system TA229005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2871487..2872127
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: LVJ72_RS13485
• Protein ID: WP_003412970.1
• Coordinates: 2871487..2871906 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: LVJ72_RS13490
• Protein ID: WP_003412975.1
• Coordinates: 2871903..2872127 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS13455 (LVJ72_13455)	2867072..2867794	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
LVJ72_RS13460 (LVJ72_13460)	2868311..2868538	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
LVJ72_RS13465 (LVJ72_13465)	2868535..2868936	+	402	WP_003412963.1	PIN domain-containing protein	-
LVJ72_RS13470 (LVJ72_13470)	2868971..2869891	-	921	WP_031664021.1	restriction endonuclease	-
LVJ72_RS13475 (LVJ72_13475)	2870232..2870477	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LVJ72_RS13480 (LVJ72_13480)	2870536..2871486	+	951	WP_003911916.1	ERCC4 domain-containing protein	-
LVJ72_RS13485 (LVJ72_13485)	2871487..2871906	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ72_RS13490 (LVJ72_13490)	2871903..2872127	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
LVJ72_RS13495 (LVJ72_13495)	2872158..2875001	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
LVJ72_RS13500 (LVJ72_13500)	2875073..2875474	-	402	WP_003412981.1	hypothetical protein	-
LVJ72_RS13505 (LVJ72_13505)	2875474..2875944	-	471	WP_003899364.1	transcription antitermination factor NusB	-
LVJ72_RS13510 (LVJ72_13510)	2875947..2876510	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T229005 WP_003412970.1 NZ_CP089777:c2871906-2871487 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T229005 NZ_CP124368:c68300-67995 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT229005 WP_003412975.1 NZ_CP089777:c2872127-2871903 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT229005 NZ_CP124368:c68520-68302 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09