Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2272963..2273589 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag LVJ72_RS10670 Protein ID WP_003410075.1
Coordinates 2273191..2273589 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag LVJ72_RS10665 Protein ID WP_003911750.1
Coordinates 2272963..2273190 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS10655 (LVJ72_10665) 2271002..2271346 - 345 WP_003410065.1 ferredoxin family protein -
LVJ72_RS10660 (LVJ72_10670) 2271535..2272704 - 1170 WP_003899126.1 ATP-binding protein -
LVJ72_RS10665 (LVJ72_10675) 2272963..2273190 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ72_RS10670 (LVJ72_10680) 2273191..2273589 + 399 WP_003410075.1 PIN domain nuclease Toxin
LVJ72_RS10675 (LVJ72_10685) 2273772..2274203 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
LVJ72_RS10680 (LVJ72_10690) 2274304..2274738 + 435 WP_003914358.1 DUF1398 domain-containing protein -
LVJ72_RS10685 (LVJ72_10695) 2275175..2275354 - 180 Protein_2112 hypothetical protein -
LVJ72_RS10690 (LVJ72_10700) 2275442..2276062 + 621 WP_003410086.1 IS110 family transposase -
LVJ72_RS10695 (LVJ72_10705) 2276016..2276606 + 591 WP_003899131.1 IS110 family transposase -
LVJ72_RS10700 (LVJ72_10710) 2276734..2277990 - 1257 WP_003410095.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T228999 WP_003410075.1 NZ_CP089777:2273191-2273589 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T228999 NZ_CP124367:3661071-3661358 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT228999 WP_003911750.1 NZ_CP089777:2272963-2273190 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT228999 NZ_CP124367:3661345-3661647 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References