228998

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2249203..2249789 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag LVJ72_RS10565 Protein ID WP_003410010.1
Coordinates 2249203..2249547 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag LVJ72_RS10570 Protein ID WP_003410014.1
Coordinates 2249541..2249789 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS10530 (LVJ72_10540) 2244909..2245508 + 600 WP_003409989.1 L-lysine exporter -
LVJ72_RS10535 (LVJ72_10545) 2245924..2246352 + 429 WP_003409992.1 cellulose-binding protein -
LVJ72_RS10540 (LVJ72_10550) 2246578..2247117 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ72_RS10545 (LVJ72_10555) 2247637..2248197 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
LVJ72_RS10550 (LVJ72_10560) 2248194..2248535 - 342 WP_003410003.1 DUF2384 domain-containing protein -
LVJ72_RS10555 (LVJ72_10565) 2248621..2248878 + 258 WP_003410006.1 hypothetical protein -
LVJ72_RS10560 (LVJ72_10570) 2248779..2249114 - 336 WP_003410009.1 dehydrogenase -
LVJ72_RS10565 (LVJ72_10575) 2249203..2249547 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
LVJ72_RS10570 (LVJ72_10580) 2249541..2249789 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ72_RS10575 (LVJ72_10585) 2249889..2252204 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ72_RS10580 (LVJ72_10590) 2252201..2252473 - 273 WP_003410017.1 DUF1490 family protein -
LVJ72_RS10585 (LVJ72_10595) 2252526..2252882 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
LVJ72_RS10590 (LVJ72_10600) 2253039..2253806 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T228998 WP_003410010.1 NZ_CP089777:c2249547-2249203 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T228998 NZ_CP124367:3580464-3580841 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT228998 WP_003410014.1 NZ_CP089777:c2249789-2249541 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT228998 NZ_CP124367:3580006-3580374 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4FAW9

References