Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mbcAT/RES-TIGR02293
Location 2247637..2248535 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name mbcT Uniprot ID P64908
Locus tag LVJ72_RS10545 Protein ID WP_003410001.1
Coordinates 2247637..2248197 (-) Length 187 a.a.

Antitoxin (Protein)


Gene name mbcA Uniprot ID P64910
Locus tag LVJ72_RS10550 Protein ID WP_003410003.1
Coordinates 2248194..2248535 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS10515 (LVJ72_10525) 2242806..2243459 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ72_RS10520 (LVJ72_10530) 2243574..2243804 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ72_RS10525 (LVJ72_10535) 2243889..2244800 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ72_RS10530 (LVJ72_10540) 2244909..2245508 + 600 WP_003409989.1 L-lysine exporter -
LVJ72_RS10535 (LVJ72_10545) 2245924..2246352 + 429 WP_003409992.1 cellulose-binding protein -
LVJ72_RS10540 (LVJ72_10550) 2246578..2247117 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ72_RS10545 (LVJ72_10555) 2247637..2248197 - 561 WP_003410001.1 RES family NAD+ phosphorylase Toxin
LVJ72_RS10550 (LVJ72_10560) 2248194..2248535 - 342 WP_003410003.1 DUF2384 domain-containing protein Antitoxin
LVJ72_RS10555 (LVJ72_10565) 2248621..2248878 + 258 WP_003410006.1 hypothetical protein -
LVJ72_RS10560 (LVJ72_10570) 2248779..2249114 - 336 WP_003410009.1 dehydrogenase -
LVJ72_RS10565 (LVJ72_10575) 2249203..2249547 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 -
LVJ72_RS10570 (LVJ72_10580) 2249541..2249789 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family -
LVJ72_RS10575 (LVJ72_10585) 2249889..2252204 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ72_RS10580 (LVJ72_10590) 2252201..2252473 - 273 WP_003410017.1 DUF1490 family protein -
LVJ72_RS10585 (LVJ72_10595) 2252526..2252882 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(25-148)

Antitoxin

Antitoxin Xre/MbcA/ParS-like, toxin-binding domain

(69-110)


Sequences

Toxin        


Download         Length: 187 a.a.        Molecular weight: 20248.70 Da        Isoelectric Point: 4.5091

>T228997 WP_003410001.1 NZ_CP089777:c2248197-2247637 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Download         Length: 561 bp

>T228997 NZ_CP124367:3480852-3481202 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 12488.33 Da        Isoelectric Point: 4.8359

>AT228997 WP_003410003.1 NZ_CP089777:c2248535-2248194 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Download         Length: 342 bp

>AT228997 NZ_CP124367:3480607-3480858 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6FKG

Antitoxin

Source ID Structure
PDB 6FKG
AlphaFold DB A0A7U4FAE4

References