Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1689967..1690583 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag LVJ72_RS07960 Protein ID WP_003407593.1
Coordinates 1690266..1690583 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag LVJ72_RS07955 Protein ID WP_003900349.1
Coordinates 1689967..1690269 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS07945 (LVJ72_07945) 1685853..1687700 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
LVJ72_RS07950 (LVJ72_07950) 1687701..1689953 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
LVJ72_RS07955 (LVJ72_07955) 1689967..1690269 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
LVJ72_RS07960 (LVJ72_07960) 1690266..1690583 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
LVJ72_RS07965 (LVJ72_07965) 1690580..1691584 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
LVJ72_RS07970 (LVJ72_07970) 1691637..1692926 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
LVJ72_RS07975 (LVJ72_07975) 1692999..1693724 - 726 WP_003898898.1 class I SAM-dependent methyltransferase -
LVJ72_RS07980 (LVJ72_07980) 1693830..1694042 - 213 WP_003898900.1 dodecin family protein -
LVJ72_RS07985 (LVJ72_07985) 1694103..1694501 + 399 WP_003900351.1 hypothetical protein -
LVJ72_RS07990 (LVJ72_07990) 1694546..1695574 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T228989 WP_003407593.1 NZ_CP089777:1690266-1690583 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T228989 NZ_CP124367:2120647-2121111 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT228989 WP_003900349.1 NZ_CP089777:1689967-1690269 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT228989 NZ_CP124367:2120312-2120647 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References