Detailed information of TA system TA228986

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1389138..1389826
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ72_RS06640
• Protein ID: WP_003406304.1
• Coordinates: 1389395..1389826 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ72_RS06635
• Protein ID: WP_003406302.1
• Coordinates: 1389138..1389398 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS06615 (LVJ72_06605)	1384715..1385539	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ72_RS06620 (LVJ72_06610)	1385544..1386725	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ72_RS06625 (LVJ72_06615)	1386802..1387902	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ72_RS06630 (LVJ72_06620)	1388073..1389062	+	990	WP_003406301.1	malate dehydrogenase	-
LVJ72_RS06635 (LVJ72_06625)	1389138..1389398	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ72_RS06640 (LVJ72_06630)	1389395..1389826	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ72_RS06645 (LVJ72_06635)	1389849..1391537	-	1689	WP_003910308.1	PE family protein	-
LVJ72_RS06650 (LVJ72_06640)	1391717..1392577	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ72_RS06655 (LVJ72_06645)	1392658..1393488	-	831	WP_031663931.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ72_RS06660 (LVJ72_06650)	1393545..1393838	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ72_RS06665 (LVJ72_06655)	1393835..1394104	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T228986 WP_003406304.1 NZ_CP089777:1389395-1389826 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T228986 NZ_CP124367:1898794-1899171 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT228986 WP_003406302.1 NZ_CP089777:1389138-1389398 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT228986 NZ_CP124367:1898337-1898717 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACTCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6