Detailed information of TA system TA228985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1244361..1244929
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ72_RS05965
• Protein ID: WP_003405865.1
• Coordinates: 1244555..1244929 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ72_RS05960
• Protein ID: WP_003405863.1
• Coordinates: 1244361..1244558 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS05940 (LVJ72_05935)	1240402..1241040	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ72_RS05945 (LVJ72_05940)	1241130..1242137	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ72_RS05950 (LVJ72_05945)	1242154..1243137	-	984	WP_003898731.1	hypothetical protein	-
LVJ72_RS05955 (LVJ72_05950)	1243200..1244273	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ72_RS05960 (LVJ72_05955)	1244361..1244558	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ72_RS05965 (LVJ72_05960)	1244555..1244929	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ72_RS05970 (LVJ72_05965)	1245132..1245830	+	699	WP_003898733.1	hypothetical protein	-
LVJ72_RS05975 (LVJ72_05970)	1245948..1246133	+	186	WP_003901093.1	hypothetical protein	-
LVJ72_RS05980 (LVJ72_05975)	1246060..1246335	-	276	WP_003405867.1	hypothetical protein	-
LVJ72_RS05985 (LVJ72_05980)	1246578..1246901	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ72_RS05990 (LVJ72_05985)	1246916..1247614	-	699	WP_031646953.1	hypothetical protein	-
LVJ72_RS05995 (LVJ72_05990)	1247648..1247857	+	210	WP_003911400.1	hypothetical protein	-
LVJ72_RS06000 (LVJ72_05995)	1247809..1248579	-	771	Protein_1182	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T228985 WP_003405865.1 NZ_CP089777:1244555-1244929 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228985 NZ_CP124367:c1752408-1752001 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228985 WP_003405863.1 NZ_CP089777:1244361-1244558 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228985 NZ_CP124367:c1752655-1752389 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07