Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1235605..1236236 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag LVJ72_RS05910 Protein ID WP_003898728.1
Coordinates 1235605..1235916 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag LVJ72_RS05915 Protein ID WP_003405836.1
Coordinates 1235916..1236236 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS05890 (LVJ72_05885) 1231086..1232510 - 1425 WP_003405805.1 class II fumarate hydratase -
LVJ72_RS05895 (LVJ72_05890) 1232541..1233629 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
LVJ72_RS05900 (LVJ72_05895) 1233685..1234329 + 645 WP_003915494.1 DUF4245 domain-containing protein -
LVJ72_RS05905 (LVJ72_05900) 1234336..1235493 - 1158 WP_003898727.1 AI-2E family transporter -
LVJ72_RS05910 (LVJ72_05905) 1235605..1235916 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
LVJ72_RS05915 (LVJ72_05910) 1235916..1236236 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
LVJ72_RS05920 (LVJ72_05915) 1236246..1237782 + 1537 Protein_1166 carboxylesterase/lipase family protein -
LVJ72_RS05925 (LVJ72_05920) 1237789..1238901 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
LVJ72_RS05930 (LVJ72_05925) 1238911..1239168 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
LVJ72_RS05935 (LVJ72_05930) 1239158..1240405 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
LVJ72_RS05940 (LVJ72_05935) 1240402..1241040 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T228984 WP_003898728.1 NZ_CP089777:c1235916-1235605 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T228984 NZ_CP124367:c829875-829624 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT228984 WP_003405836.1 NZ_CP089777:c1236236-1235916 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT228984 NZ_CP124367:c829627-829373 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References