Detailed information of TA system TA022898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 13502..14054
• Replicon: plasmid pMPOP01
• Accession: NC_010727
• Organism: Methylobacterium populi BJ001

Toxin (Protein)

• Gene name: parE
• Uniprot ID: B1ZM49
• Locus tag: MPOP_RS26865
• Protein ID: WP_012457143.1
• Coordinates: 13502..13792 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: B1ZM50
• Locus tag: MPOP_RS26870
• Protein ID: WP_012457144.1
• Coordinates: 13785..14054 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPOP_RS26840	9180..9545	+	366	WP_041372211.1	hypothetical protein	-
MPOP_RS26845	9542..10015	+	474	WP_012457139.1	hypothetical protein	-
MPOP_RS26850	10351..10635	-	285	WP_012457140.1	integration host factor subunit beta	-
MPOP_RS26855	11365..12129	+	765	WP_012457141.1	metallophosphoesterase	-
MPOP_RS26860	12217..12960	-	744	WP_012457142.1	GntR family transcriptional regulator	-
MPOP_RS26865	13502..13792	-	291	WP_012457143.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MPOP_RS26870	13785..14054	-	270	WP_012457144.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
MPOP_RS26875	14313..14999	+	687	WP_012457145.1	ParA family protein	-
MPOP_RS28055	14996..15364	+	369	WP_158022323.1	stability/partitioning determinant	-
MPOP_RS28610	15473..15637	-	165	WP_158022324.1	hypothetical protein	-
MPOP_RS26885	15793..16230	-	438	WP_012457147.1	hypothetical protein	-
MPOP_RS26890	16235..16816	-	582	WP_012457148.1	recombinase family protein	-
MPOP_RS26895	17132..17590	-	459	Protein_19	FCD domain-containing protein	-
MPOP_RS26900	17647..18399	-	753	WP_012457137.1	IS6-like element ISMpo1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..25164	25164
	-	flank	IS/Tn	-	-	17647..18399	752

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-89)

Antitoxin

• Antitoxin ParD (3-74)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10556.04 Da
• Isoelectric Point: 6.0642

>T22898 WP_012457143.1 NC_010727:c13792-13502 [Methylorubrum populi BJ001]
VGSYQLTGRADADILDVFLYGLTEFGMAQAERYQGILEHTFQLLADNPRMGRAAEVIGAGVRRHECGAHVILYEEATDGV
LILAVVHSRSVRRLSL

Nucleotide

• Length: 291 bp

>T22898 NC_010727:c13792-13502 [Methylorubrum populi BJ001]
GTGGGTAGCTACCAGCTCACTGGCCGCGCCGACGCCGACATCCTCGACGTGTTCCTCTACGGCCTCACCGAATTTGGGAT
GGCCCAGGCCGAGCGCTATCAGGGCATCCTGGAGCACACCTTTCAGCTCCTGGCCGACAATCCTCGTATGGGCCGGGCTG
CAGAGGTGATCGGGGCAGGGGTCCGGCGCCACGAATGCGGCGCACACGTCATCCTCTACGAGGAGGCAACGGACGGCGTC
CTGATCCTCGCGGTCGTCCACAGCAGGAGCGTCCGGCGGCTTTCGCTGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10121.35 Da
• Isoelectric Point: 7.0158

>AT22898 WP_012457144.1 NC_010727:c14054-13785 [Methylorubrum populi BJ001]
MATMTISLPDPMKEWIEAQIRQGDYASTSDYVRDLVRRDRERRAHPELTIDDLRRIVDDSRASGISRRSISDIMAEAKEI
ASARGTSRG

Nucleotide

• Length: 270 bp

>AT22898 NC_010727:c14054-13785 [Methylorubrum populi BJ001]
ATGGCGACGATGACGATCTCCCTACCCGACCCAATGAAGGAATGGATCGAGGCCCAGATCAGGCAAGGCGATTACGCCAG
CACGAGCGACTATGTGCGTGACCTCGTGCGTCGTGACCGCGAGCGCCGGGCTCACCCCGAACTGACGATCGACGATCTCC
GCCGCATCGTGGACGACTCCCGCGCCAGCGGCATCAGCCGCCGCAGCATCTCGGACATCATGGCCGAGGCCAAGGAGATT
GCGAGCGCTCGCGGCACTTCGCGTGGGTAG

Structures

Toxin

• AlphaFold DB: B1ZM49

Antitoxin

• AlphaFold DB: B1ZM50