Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 715406..716031 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID G0TQA0
Locus tag LVJ72_RS03260 Protein ID WP_003403218.1
Coordinates 715630..716031 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ36
Locus tag LVJ72_RS03255 Protein ID WP_003403213.1
Coordinates 715406..715633 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS03230 (LVJ72_03225) 710584..710805 - 222 WP_003898525.1 DUF4926 domain-containing protein -
LVJ72_RS03235 (LVJ72_03230) 710947..711552 + 606 WP_003898526.1 hypothetical protein -
LVJ72_RS03240 (LVJ72_03235) 711571..714138 - 2568 WP_003900188.1 SEC-C metal-binding domain-containing protein -
LVJ72_RS03245 (LVJ72_03240) 714222..714971 + 750 WP_003898528.1 hypothetical protein -
LVJ72_RS03250 (LVJ72_03245) 714968..715210 + 243 WP_003403210.1 hypothetical protein -
LVJ72_RS03255 (LVJ72_03250) 715406..715633 + 228 WP_003403213.1 antitoxin VapB29 Antitoxin
LVJ72_RS03260 (LVJ72_03255) 715630..716031 + 402 WP_003403218.1 type II toxin-antitoxin system ribonuclease VapC29 Toxin
LVJ72_RS03265 (LVJ72_03260) 716160..716243 + 84 Protein_647 galactose-1-phosphate uridylyltransferase -
LVJ72_RS03270 (LVJ72_03265) 716262..717343 + 1082 Protein_648 galactose-1-phosphate uridylyltransferase -
LVJ72_RS03275 (LVJ72_03270) 717340..718431 + 1092 WP_003906403.1 galactokinase -
LVJ72_RS03280 (LVJ72_03275) 718826..719890 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ72_RS03285 (LVJ72_03280) 719994..720941 + 948 WP_003403232.1 DUF732 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-120)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 13937.76 Da        Isoelectric Point: 5.7441

>T228973 WP_003403218.1 NZ_CP089777:715630-716031 [Mycobacterium tuberculosis]
VTVLLDANVLIALVVAEHVHHDAAADWLMASDTGFATCPMTQGSLVRFLVRSGQSAAAARDVVSAVQCTSRHEFWPDALS
FAGVEVAGVVGHRQVTDAYLAQLARSHDGQLATLDSGLAHLHGDVAVLIPTTT

Download         Length: 402 bp

>T228973 NZ_CP124359:3631511-3631729 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8163.17 Da        Isoelectric Point: 5.6307

>AT228973 WP_003403213.1 NZ_CP089777:715406-715633 [Mycobacterium tuberculosis]
VRTTIDLPQDLHKQALAIARDTHRTLSETVADLMRRGLAANRPTALSSDPRTGLPLVSVGTVVTSEDVRSLEDEQ

Download         Length: 228 bp

>AT228973 NZ_CP124359:3631111-3631485 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQA0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSC9

References