Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 699462..700108 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID O07783
Locus tag LVJ72_RS03140 Protein ID WP_003403122.1
Coordinates 699462..699854 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ86
Locus tag LVJ72_RS03145 Protein ID WP_003403125.1
Coordinates 699851..700108 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS03125 (LVJ72_03120) 695124..696650 + 1527 WP_003403112.1 virulence factor Mce family protein -
LVJ72_RS03130 (LVJ72_03125) 696713..697855 + 1143 WP_003911253.1 virulence factor Mce family protein -
LVJ72_RS03135 (LVJ72_03130) 697860..699410 + 1551 WP_003403119.1 MlaD family protein -
LVJ72_RS03140 (LVJ72_03135) 699462..699854 - 393 WP_003403122.1 type II toxin-antitoxin system toxin ribonuclease C4 Toxin
LVJ72_RS03145 (LVJ72_03140) 699851..700108 - 258 WP_003403125.1 type II toxin-antitoxin system antitoxin VapB4 Antitoxin
LVJ72_RS03150 (LVJ72_03145) 700291..701526 - 1236 WP_070898222.1 ATP-binding protein -
LVJ72_RS03155 (LVJ72_03150) 701777..702190 - 414 WP_031705732.1 PIN domain-containing protein -
LVJ72_RS03160 (LVJ72_03155) 702187..702423 - 237 WP_003403139.1 type II toxin-antitoxin system antitoxin VapB27 -
LVJ72_RS03165 (LVJ72_03160) 702527..703033 - 507 WP_003900973.1 two component system sensor kinase HK1 -
LVJ72_RS03170 (LVJ72_03165) 703147..703677 - 531 WP_003917320.1 two component system sensor kinase HK2 -
LVJ72_RS03175 (LVJ72_03170) 703661..704356 - 696 WP_003918099.1 two-component system response regulator TcrA -
LVJ72_RS03180 (LVJ72_03175) 704479..704790 + 312 WP_003403164.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(8-122)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(3-53)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14080.08 Da        Isoelectric Point: 4.6558

>T228970 WP_003403122.1 NZ_CP089777:c699854-699462 [Mycobacterium tuberculosis]
VNVRRALADTSVFIGIEATRFDPDRFAGYEWGVSVVTLGELRLGVLQASGPEAAARRLSTYQLAQRFEPLGIDEAVSEAW
ALLVSKLRAAKLRVPINDSWIAATAVAHGIAILTQDNDYAAMPDVEVITI

Download         Length: 393 bp

>T228970 NZ_CP124359:2799755-2799862 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9681.06 Da        Isoelectric Point: 7.0175

>AT228970 WP_003403125.1 NZ_CP089777:c700108-699851 [Mycobacterium tuberculosis]
MSATIPARDLRNHTAEVLRRVAAGEEIEVLKDNRPVARIVPLKRRRQWLPAAEVIGELVRLGPDTTNLGEELRETLTQTT
DDVRW

Download         Length: 258 bp

>AT228970 NZ_CP124359:c2799698-2799643 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F8V4

Antitoxin

Source ID Structure
AlphaFold DB G0TQ86

References