Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 645984..646660 Replicon chromosome
Accession NZ_CP089777
Organism Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag LVJ72_RS02905 Protein ID WP_003898500.1
Coordinates 645984..646397 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag LVJ72_RS02910 Protein ID WP_003402915.1
Coordinates 646394..646660 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ72_RS02875 (LVJ72_02870) 641329..641631 - 303 WP_003402896.1 DUF3349 domain-containing protein -
LVJ72_RS02880 (LVJ72_02875) 641691..641969 - 279 WP_003402901.1 hypothetical protein -
LVJ72_RS02885 (LVJ72_02880) 641966..643219 - 1254 WP_003402904.1 inorganic phosphate transporter -
LVJ72_RS02890 (LVJ72_02885) 643339..643725 - 387 WP_003402907.1 VOC family protein -
LVJ72_RS02895 (LVJ72_02890) 643788..644672 - 885 WP_003402909.1 SDR family oxidoreductase -
LVJ72_RS02900 (LVJ72_02895) 644768..645670 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
LVJ72_RS02905 (LVJ72_02900) 645984..646397 - 414 WP_003898500.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ72_RS02910 (LVJ72_02905) 646394..646660 - 267 WP_003402915.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LVJ72_RS02915 (LVJ72_02910) 646852..648567 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
LVJ72_RS02920 (LVJ72_02915) 648645..650249 + 1605 WP_003402920.1 amidohydrolase -
LVJ72_RS02925 (LVJ72_02920) 650246..651226 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T228968 WP_003898500.1 NZ_CP089777:c646397-645984 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T228968 NZ_CP124359:2266996-2267098 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT228968 WP_003402915.1 NZ_CP089777:c646660-646394 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT228968 NZ_CP124359:c2267136-2266868 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATATTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References