Detailed information of TA system TA228967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 449917..450928
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: L7N5R2
• Locus tag: LVJ72_RS01945
• Protein ID: WP_003401859.1
• Coordinates: 449917..450510 (-)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: LVJ72_RS01950
• Protein ID: WP_003401860.1
• Coordinates: 450539..450928 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS01925 (LVJ72_01925)	445566..446948	+	1383	WP_003898415.1	magnesium transporter	-
LVJ72_RS01930 (LVJ72_01930)	446960..447994	-	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
LVJ72_RS01935 (LVJ72_01935)	448090..448773	+	684	WP_003401857.1	VTT domain-containing protein	-
LVJ72_RS01940 (LVJ72_01940)	448762..449892	-	1131	WP_031705762.1	glycoside hydrolase family 76 protein	-
LVJ72_RS01945 (LVJ72_01945)	449917..450510	-	594	WP_003401859.1	zeta toxin family protein	Toxin
LVJ72_RS01950 (LVJ72_01950)	450539..450928	-	390	WP_003401860.1	hypothetical protein	Antitoxin
LVJ72_RS01955 (LVJ72_01955)	451009..452220	-	1212	WP_003906353.1	VWA domain-containing protein	-
LVJ72_RS01960 (LVJ72_01960)	452226..452828	-	603	WP_003898417.1	SRPBCC family protein	-
LVJ72_RS01965 (LVJ72_01965)	452842..453738	-	897	WP_003898418.1	MoxR family ATPase	-
LVJ72_RS01970 (LVJ72_01970)	453735..454328	-	594	WP_003401864.1	NTP transferase domain-containing protein	-
LVJ72_RS01975 (LVJ72_01975)	454325..455080	-	756	WP_003401865.1	XdhC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21344.53 Da
• Isoelectric Point: 10.5018

>T228967 WP_003401859.1 NZ_CP089777:c450510-449917 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDNSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T228967 NZ_CP124359:c2023920-2023546 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT228967 WP_003401860.1 NZ_CP089777:c450928-450539 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT228967 NZ_CP124359:c2024377-2024009 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A7U4BRU8

Antitoxin

• AlphaFold DB: A0A7U4BRT0