Detailed information of TA system TA228964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 369139..369665
• Replicon: chromosome
• Accession: NZ_CP089777
• Organism: Mycobacterium tuberculosis strain I0003270-1

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: LVJ72_RS01595
• Protein ID: WP_003401560.1
• Coordinates: 369363..369665 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: P9WJ08
• Locus tag: LVJ72_RS01590
• Protein ID: WP_003401555.1
• Coordinates: 369139..369366 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ72_RS01575 (LVJ72_01575)	364832..365635	-	804	WP_003401540.1	Stf0 family sulphotransferase	-
LVJ72_RS01580 (LVJ72_01580)	365645..367042	-	1398	WP_003401544.1	sulfatase	-
LVJ72_RS01585 (LVJ72_01585)	367221..368996	+	1776	WP_010886074.1	PE family protein	-
LVJ72_RS01590 (LVJ72_01590)	369139..369366	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	Antitoxin
LVJ72_RS01595 (LVJ72_01595)	369363..369665	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
LVJ72_RS01600 (LVJ72_01600)	369713..369934	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	-
LVJ72_RS01605 (LVJ72_01605)	369931..370356	+	426	WP_003401566.1	PIN domain nuclease	-
LVJ72_RS01610 (LVJ72_01610)	370492..371124	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
LVJ72_RS01615 (LVJ72_01615)	371121..372029	+	909	WP_003900117.1	protochlorophyllide reductase	-
LVJ72_RS01620 (LVJ72_01620)	372037..372627	-	591	WP_229298012.1	hypothetical protein	-
LVJ72_RS01625 (LVJ72_01625)	372620..372670	-	51	Protein_321	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T228964 WP_003401560.1 NZ_CP089777:369363-369665 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T228964 NZ_CP124359:976016-976423 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8130.21 Da
• Isoelectric Point: 7.0084

>AT228964 WP_003401555.1 NZ_CP089777:369139-369366 [Mycobacterium tuberculosis]
MTKEKISVTVDAAVLAAIDADARAAGLNRSEMIEQALRNEHLRVALRDYTAKTVPALDIDAYAQRVYQANRAAGS

Nucleotide

• Length: 228 bp

>AT228964 NZ_CP124359:975769-976035 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4F8T1