Detailed information of TA system TA228962

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3820484..3821169
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: LVJ71_RS17990
• Protein ID: WP_003417998.1
• Coordinates: 3820759..3821169 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LVJ71_RS17985
• Protein ID: WP_003912220.1
• Coordinates: 3820484..3820762 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS17965 (LVJ71_17965)	3816473..3818074	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
LVJ71_RS17970 (LVJ71_17970)	3818091..3818795	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
LVJ71_RS17975 (LVJ71_17975)	3818913..3819479	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
LVJ71_RS17980 (LVJ71_17980)	3819541..3820428	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
LVJ71_RS17985 (LVJ71_17985)	3820484..3820762	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LVJ71_RS17990 (LVJ71_17990)	3820759..3821169	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ71_RS17995 (LVJ71_17995)	3821202..3822938	-	1737	WP_003418002.1	cholesterol oxidase	-
LVJ71_RS18000 (LVJ71_18000)	3822994..3824121	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
LVJ71_RS18005 (LVJ71_18005)	3824141..3825730	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T228962 WP_003417998.1 NZ_CP089776:3820759-3821169 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T228962 NZ_CP124359:645572-645886 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT228962 WP_003912220.1 NZ_CP089776:3820484-3820762 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT228962 NZ_CP124359:645883-646299 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: G0TIR9