Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3174999..3175686 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag LVJ71_RS15165 Protein ID WP_003414624.1
Coordinates 3175243..3175686 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag LVJ71_RS15160 Protein ID WP_003414620.1
Coordinates 3174999..3175256 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS15140 (LVJ71_15140) 3170319..3171173 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ71_RS15145 (LVJ71_15145) 3171229..3172392 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ71_RS15150 (LVJ71_15150) 3172409..3173623 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ71_RS15155 (LVJ71_15155) 3173631..3174872 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
LVJ71_RS15160 (LVJ71_15160) 3174999..3175256 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
LVJ71_RS15165 (LVJ71_15165) 3175243..3175686 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ71_RS15170 (LVJ71_15170) 3175766..3176428 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
LVJ71_RS15175 (LVJ71_15175) 3176524..3176715 + 192 WP_003414632.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
LVJ71_RS15180 (LVJ71_15180) 3177047..3178795 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
LVJ71_RS15185 (LVJ71_15185) 3178891..3179472 + 582 WP_003414644.1 fasciclin domain-containing protein -
LVJ71_RS15190 (LVJ71_15190) 3179572..3179838 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T228957 WP_003414624.1 NZ_CP089776:3175243-3175686 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T228957 NZ_CP124358:c133835-133530 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT228957 WP_003414620.1 NZ_CP089776:3174999-3175256 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT228957 NZ_CP124358:c134055-133837 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References