Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2860650..2861281 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag LVJ71_RS13455 Protein ID WP_003413174.1
Coordinates 2860904..2861281 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag LVJ71_RS13450 Protein ID WP_003413167.1
Coordinates 2860650..2860907 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS13420 (LVJ71_13420) 2856564..2856878 + 315 WP_009937839.1 hypothetical protein -
LVJ71_RS13425 (LVJ71_13425) 2857174..2858385 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
LVJ71_RS13430 (LVJ71_13430) 2858512..2859171 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ71_RS13435 (LVJ71_13435) 2859168..2859830 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ71_RS13440 (LVJ71_13440) 2859827..2860105 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ71_RS13445 (LVJ71_13445) 2860198..2860611 + 414 WP_003912787.1 PIN domain nuclease -
LVJ71_RS13450 (LVJ71_13450) 2860650..2860907 + 258 WP_003413167.1 CopG family transcriptional regulator Antitoxin
LVJ71_RS13455 (LVJ71_13455) 2860904..2861281 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ71_RS13460 (LVJ71_13460) 2861297..2861671 - 375 WP_003413177.1 hypothetical protein -
LVJ71_RS13465 (LVJ71_13465) 2861771..2862166 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
LVJ71_RS13470 (LVJ71_13470) 2862163..2862408 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
LVJ71_RS13475 (LVJ71_13475) 2862819..2863238 - 420 WP_003413190.1 A24 family peptidase -
LVJ71_RS13480 (LVJ71_13480) 2863250..2864059 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ71_RS13485 (LVJ71_13485) 2864056..2865309 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ71_RS13490 (LVJ71_13490) 2865302..2865814 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T228947 WP_003413174.1 NZ_CP089776:2860904-2861281 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T228947 NZ_CP124357:2795406-2795513 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT228947 WP_003413167.1 NZ_CP089776:2860650-2860907 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT228947 NZ_CP124357:c2795349-2795294 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References