Detailed information of TA system TA228943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 2396921..2397450
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TMR4
• Locus tag: LVJ71_RS11250
• Protein ID: WP_003411124.1
• Coordinates: 2396921..2397238 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P9WJ74
• Locus tag: LVJ71_RS11255
• Protein ID: WP_003411127.1
• Coordinates: 2397235..2397450 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS11220 (LVJ71_11230)	2392058..2393134	+	1077	WP_003411110.1	hypothetical protein	-
LVJ71_RS11225 (LVJ71_11235)	2393131..2393412	+	282	WP_003411112.1	DUF5703 family protein	-
LVJ71_RS11230 (LVJ71_11240)	2393448..2394521	+	1074	WP_003411116.1	quinone-dependent dihydroorotate dehydrogenase	-
LVJ71_RS11235 (LVJ71_11245)	2394526..2395056	-	531	WP_003411119.1	YbhB/YbcL family Raf kinase inhibitor-like protein	-
LVJ71_RS11240 (LVJ71_11250)	2395104..2396450	-	1347	WP_003411121.1	M20/M25/M40 family metallo-hydrolase	-
LVJ71_RS11250 (LVJ71_11260)	2396921..2397238	-	318	WP_003411124.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ71_RS11255 (LVJ71_11265)	2397235..2397450	-	216	WP_003411127.1	antitoxin ParD2	Antitoxin
LVJ71_RS11260 (LVJ71_11270)	2397705..2398763	+	1059	WP_003411129.1	phosphoribosyltransferase family protein	-
LVJ71_RS11265 (LVJ71_11275)	2398893..2399249	-	357	WP_003411130.1	hypothetical protein	-
LVJ71_RS11270 (LVJ71_11280)	2399344..2400126	-	783	WP_003411131.1	cell wall synthesis protein Wag31	-
LVJ71_RS11275 (LVJ71_11285)	2400394..2400684	-	291	WP_003900476.1	YggT family protein	-
LVJ71_RS11280 (LVJ71_11290)	2400846..2401502	-	657	WP_003411133.1	cell division protein SepF	-
LVJ71_RS11285 (LVJ71_11295)	2401568..2402344	-	777	WP_003411137.1	YggS family pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-92)

Antitoxin

• Conserved hypothetical protein CHP02574, addiction module (11-69)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12312.88 Da
• Isoelectric Point: 6.4831

>T228943 WP_003411124.1 NZ_CP089776:c2397238-2396921 [Mycobacterium tuberculosis]
MTRRLRVHNGVEDDLFEAFSYYADAAPDQIDRLYNLFVDAVTKRIPQAPNAFAPLFKHYRHIYLRPFRYYVAYRTTDEAI
DILAVRHGMENPNAVEAEISGRTFE

Nucleotide

• Length: 318 bp

>T228943 NZ_CP124357:2000949-2001203 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7880.70 Da
• Isoelectric Point: 4.4055

>AT228943 WP_003411127.1 NZ_CP089776:c2397450-2397235 [Mycobacterium tuberculosis]
VVVNRALLASVDALSRDEQIELVEHINGNLAEGMHISEANQALIEARANDTDDAHWSTIDDFDKRIRARLG

Nucleotide

• Length: 216 bp

>AT228943 NZ_CP124357:2000701-2000952 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TMR4

Antitoxin

• AlphaFold DB: A0A7U4FAJ4