Detailed information of TA system TA022894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 3409669..3410255
• Replicon: chromosome
• Accession: NC_010725
• Organism: Methylobacterium populi BJ001

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B1ZHK9
• Locus tag: MPOP_RS15795
• Protein ID: WP_012455044.1
• Coordinates: 3409953..3410255 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: MPOP_RS15790
• Protein ID: WP_012455043.1
• Coordinates: 3409669..3409956 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPOP_RS15770	3405659..3406384	+	726	WP_131801261.1	hypothetical protein	-
MPOP_RS15775	3406519..3407790	-	1272	WP_012455040.1	tyrosine--tRNA ligase	-
MPOP_RS15780	3407929..3409062	+	1134	WP_012455041.1	anhydro-N-acetylmuramic acid kinase	-
MPOP_RS15785	3409059..3409664	+	606	WP_012455042.1	DUF3578 domain-containing protein	-
MPOP_RS15790	3409669..3409956	-	288	WP_012455043.1	putative addiction module antidote protein	Antitoxin
MPOP_RS15795	3409953..3410255	-	303	WP_012455044.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MPOP_RS15800	3410388..3411488	+	1101	WP_012455045.1	chorismate synthase	-
MPOP_RS15805	3411524..3412600	+	1077	WP_012455046.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
MPOP_RS15810	3412700..3412933	+	234	WP_012455047.1	hypothetical protein	-
MPOP_RS15815	3412958..3413887	-	930	WP_041372038.1	histone deacetylase family protein	-
MPOP_RS15820	3414540..3415022	+	483	WP_012455049.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-94)

Antitoxin

• Uncharacterized protein HI1420 (3-90)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11272.06 Da
• Isoelectric Point: 10.6147

>T22894 WP_012455044.1 NC_010725:c3410255-3409953 [Methylorubrum populi BJ001]
MPEIIRTRVFADWLRGLRDDRAKARIALRIDRLALGHEGDSKPVGGGVSELRIDYGPGYRIYFKYRDGTLIILLCGGDKS
SQKRDITRARSLAATLKEKP

Nucleotide

• Length: 303 bp

>T22894 NC_010725:c3410255-3409953 [Methylorubrum populi BJ001]
ATGCCGGAGATTATCCGAACCCGCGTGTTCGCGGACTGGCTCCGAGGACTTCGAGACGATCGTGCGAAGGCACGGATCGC
CTTGCGGATCGACCGCCTTGCACTTGGCCATGAAGGTGATTCGAAGCCTGTCGGCGGAGGCGTCAGCGAACTGAGGATCG
ATTACGGGCCGGGTTACCGGATCTACTTCAAGTACCGCGACGGGACGCTCATCATCCTTCTTTGTGGAGGGGACAAGTCC
AGCCAGAAGCGCGACATCACCCGTGCGCGCTCCCTCGCGGCGACGCTGAAAGAAAAGCCATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10195.38 Da
• Isoelectric Point: 4.3313

>AT22894 WP_012455043.1 NC_010725:c3409956-3409669 [Methylorubrum populi BJ001]
MTELSPYDSAEFLDSDEAVAEYLAAAIEEGDAALFRRALGVAARARGMTQIARETGLSRESLYRALSDDGNPSLDTALRV
LNALNVRLTASVVPH

Nucleotide

• Length: 288 bp

>AT22894 NC_010725:c3409956-3409669 [Methylorubrum populi BJ001]
ATGACAGAGTTGAGCCCCTACGACTCGGCCGAATTCCTCGACAGCGACGAGGCCGTCGCTGAGTATCTCGCTGCAGCCAT
CGAGGAAGGCGATGCCGCGCTGTTTCGTCGGGCGCTTGGCGTGGCGGCGCGGGCCCGCGGCATGACCCAGATCGCCCGCG
AAACGGGCTTGTCGCGGGAAAGCCTCTATCGGGCGCTGTCGGATGACGGCAATCCCAGCCTCGACACGGCGTTGCGCGTC
CTCAACGCTCTGAACGTGCGCCTTACGGCCTCCGTGGTGCCTCACTGA

Structures

Toxin

• AlphaFold DB: B1ZHK9