Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2225554..2226140 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag LVJ71_RS10460 Protein ID WP_003912702.1
Coordinates 2225554..2225898 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag LVJ71_RS10465 Protein ID WP_003410014.1
Coordinates 2225892..2226140 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS10425 (LVJ71_10435) 2221260..2221859 + 600 WP_003409989.1 L-lysine exporter -
LVJ71_RS10430 (LVJ71_10440) 2222275..2222703 + 429 WP_003409992.1 cellulose-binding protein -
LVJ71_RS10435 (LVJ71_10445) 2222929..2223468 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
LVJ71_RS10440 (LVJ71_10450) 2223988..2224548 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
LVJ71_RS10445 (LVJ71_10455) 2224545..2224886 - 342 WP_003410003.1 DUF2384 domain-containing protein -
LVJ71_RS10450 (LVJ71_10460) 2224972..2225229 + 258 WP_003410006.1 hypothetical protein -
LVJ71_RS10455 (LVJ71_10465) 2225130..2225465 - 336 WP_003410009.1 dehydrogenase -
LVJ71_RS10460 (LVJ71_10470) 2225554..2225898 - 345 WP_003912702.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
LVJ71_RS10465 (LVJ71_10475) 2225892..2226140 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ71_RS10470 (LVJ71_10480) 2226240..2228555 - 2316 WP_003899120.1 cation transporter ATPase CptG -
LVJ71_RS10475 (LVJ71_10485) 2228552..2228824 - 273 WP_003410017.1 DUF1490 family protein -
LVJ71_RS10480 (LVJ71_10490) 2228877..2229233 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
LVJ71_RS10485 (LVJ71_10495) 2229390..2230157 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12260.15 Da        Isoelectric Point: 9.8887

>T228938 WP_003912702.1 NZ_CP089776:c2225898-2225554 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPSNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T228938 NZ_CP124357:c605509-605045 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT228938 WP_003410014.1 NZ_CP089776:c2226140-2225892 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT228938 NZ_CP124357:c605844-605509 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References