Detailed information of TA system TA228935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 2194929..2195473
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: parE
• Uniprot ID: G0TLU9
• Locus tag: LVJ71_RS10275
• Protein ID: WP_003409896.1
• Coordinates: 2194929..2195225 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: P67299
• Locus tag: LVJ71_RS10280
• Protein ID: WP_003409899.1
• Coordinates: 2195222..2195473 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS10220 (LVJ71_10230)	2189962..2190312	-	351	WP_003899096.1	hypothetical protein	-
LVJ71_RS10225 (LVJ71_10235)	2190323..2191225	-	903	WP_003899097.1	hypothetical protein	-
LVJ71_RS10230 (LVJ71_10240)	2191246..2191437	-	192	WP_003409876.1	hypothetical protein	-
LVJ71_RS10235 (LVJ71_10245)	2191438..2191734	-	297	WP_003409877.1	hypothetical protein	-
LVJ71_RS10240 (LVJ71_10250)	2191974..2192189	+	216	WP_003409878.1	antitoxin	-
LVJ71_RS10245 (LVJ71_10255)	2192186..2192497	+	312	WP_003409881.1	type II toxin-antitoxin system VapC family toxin	-
LVJ71_RS10250 (LVJ71_10260)	2192471..2192992	-	522	WP_003904745.1	hypothetical protein	-
LVJ71_RS10255 (LVJ71_10265)	2192967..2193344	+	378	WP_010886136.1	type II toxin-antitoxin system toxin HigB	-
LVJ71_RS10260 (LVJ71_10270)	2193386..2193835	+	450	WP_003913306.1	type II toxin-antitoxin system antitoxin HigA	-
LVJ71_RS10265 (LVJ71_10275)	2193832..2194377	+	546	WP_003409891.1	SecB-like chaperone	-
LVJ71_RS10270 (LVJ71_10280)	2194266..2194880	-	615	WP_003901296.1	hypothetical protein	-
LVJ71_RS10275 (LVJ71_10285)	2194929..2195225	-	297	WP_003409896.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ71_RS10280 (LVJ71_10290)	2195222..2195473	-	252	WP_003409899.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LVJ71_RS10285 (LVJ71_10295)	2195460..2195954	+	495	WP_003899099.1	hypothetical protein	-
LVJ71_RS10290 (LVJ71_10300)	2196114..2196521	-	408	WP_003409913.1	type II toxin-antitoxin system VapC family toxin	-
LVJ71_RS10295 (LVJ71_10305)	2196525..2196797	-	273	WP_003899100.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LVJ71_RS10300 (LVJ71_10310)	2196830..2198050	-	1221	WP_003409919.1	TetR family transcriptional regulator Mce3R	-
LVJ71_RS10305 (LVJ71_10315)	2198949..2199746	+	798	WP_003899101.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-93)

Antitoxin

• Antitoxin ParD (2-80)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11268.64 Da
• Isoelectric Point: 6.8604

>T228935 WP_003409896.1 NZ_CP089776:c2195225-2194929 [Mycobacterium tuberculosis]
VSSRYLLSPAAQAHLEEIWDCTYDRWGVDQAEQYLRELQHAIDRAAANPRIGRACDEIRPGYRKLSAGSHTLFYRVTGEG
TIDVVRVLHQRMDVDRNL

Nucleotide

• Length: 297 bp

>T228935 NZ_CP124357:188058-188165 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9207.17 Da
• Isoelectric Point: 7.0150

>AT228935 WP_003409899.1 NZ_CP089776:c2195473-2195222 [Mycobacterium tuberculosis]
VGKNTSFVLDEHYSAFIDGEIAAGRYRSASEVIRSALRLLEDRETQLRALREALEAGERSGSSTPFDFDGFLGRKRADAS
RGR

Nucleotide

• Length: 252 bp

>AT228935 NZ_CP124357:c188009-187944 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: G0TLU9

Antitoxin

• AlphaFold DB: A0A7U4BUZ2