Detailed information of TA system TA228933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2185892..2186595
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: LVJ71_RS10185
• Protein ID: WP_003409778.1
• Coordinates: 2185892..2186221 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: LVJ71_RS10190
• Protein ID: WP_003409780.1
• Coordinates: 2186218..2186595 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS10165 (LVJ71_10175)	2182275..2183345	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
LVJ71_RS10170 (LVJ71_10180)	2183342..2183857	+	516	WP_003409718.1	flavin reductase family protein	-
LVJ71_RS10175 (LVJ71_10185)	2183854..2184915	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LVJ71_RS10180 (LVJ71_10190)	2184912..2185682	+	771	WP_003409775.1	SDR family oxidoreductase	-
LVJ71_RS10185 (LVJ71_10195)	2185892..2186221	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVJ71_RS10190 (LVJ71_10200)	2186218..2186595	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
LVJ71_RS10195 (LVJ71_10205)	2186592..2187182	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
LVJ71_RS10200 (LVJ71_10210)	2187237..2188601	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
LVJ71_RS10205 (LVJ71_10215)	2188756..2189208	-	453	WP_003899095.1	lipoprotein	-
LVJ71_RS10210 (LVJ71_10220)	2189272..2189673	+	402	WP_003409869.1	hypothetical protein	-
LVJ71_RS10215 (LVJ71_10225)	2189666..2189848	-	183	WP_003409870.1	hypothetical protein	-
LVJ71_RS10220 (LVJ71_10230)	2189962..2190312	-	351	WP_003899096.1	hypothetical protein	-
LVJ71_RS10225 (LVJ71_10235)	2190323..2191225	-	903	WP_003899097.1	hypothetical protein	-
LVJ71_RS10230 (LVJ71_10240)	2191246..2191437	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T228933 WP_003409778.1 NZ_CP089776:c2186221-2185892 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T228933 NZ_CP124356:c138571-138266 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT228933 WP_003409780.1 NZ_CP089776:c2186595-2186218 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT228933 NZ_CP124356:c138791-138573 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8