Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1775705..1776318 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag LVJ71_RS08345 Protein ID WP_003407786.1
Coordinates 1775914..1776318 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag LVJ71_RS08340 Protein ID WP_009935474.1
Coordinates 1775705..1775908 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS08310 (LVJ71_08315) 1771110..1771490 + 381 WP_003407768.1 fumarate reductase subunit C -
LVJ71_RS08315 (LVJ71_08320) 1771487..1771864 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
LVJ71_RS08320 (LVJ71_08325) 1771932..1772540 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
LVJ71_RS08325 (LVJ71_08330) 1772724..1773872 + 1149 Protein_1642 MMPL family transporter -
LVJ71_RS08330 (LVJ71_08335) 1773882..1774328 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
LVJ71_RS08335 (LVJ71_08340) 1774363..1775652 + 1290 WP_003407781.1 threonine ammonia-lyase -
LVJ71_RS08340 (LVJ71_08345) 1775705..1775908 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ71_RS08345 (LVJ71_08350) 1775914..1776318 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
LVJ71_RS08350 (LVJ71_08355) 1776335..1778077 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
LVJ71_RS08355 (LVJ71_08360) 1778070..1780367 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T228930 WP_003407786.1 NZ_CP089776:1775914-1776318 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T228930 NZ_CP124355:4360914-4361216 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT228930 WP_009935474.1 NZ_CP089776:1775705-1775908 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT228930 NZ_CP124355:4361252-4361593 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References