Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1695849..1696465 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag LVJ71_RS07990 Protein ID WP_003407593.1
Coordinates 1696148..1696465 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag LVJ71_RS07985 Protein ID WP_003900349.1
Coordinates 1695849..1696151 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS07975 (LVJ71_07975) 1691735..1693582 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
LVJ71_RS07980 (LVJ71_07980) 1693583..1695835 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
LVJ71_RS07985 (LVJ71_07985) 1695849..1696151 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
LVJ71_RS07990 (LVJ71_07990) 1696148..1696465 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
LVJ71_RS07995 (LVJ71_07995) 1696462..1697466 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
LVJ71_RS08000 (LVJ71_08000) 1697519..1698808 + 1290 WP_003407599.1 serine hydrolase domain-containing protein -
LVJ71_RS08005 (LVJ71_08005) 1698881..1699606 - 726 WP_265114282.1 class I SAM-dependent methyltransferase -
LVJ71_RS08010 (LVJ71_08010) 1699712..1699924 - 213 WP_003898900.1 dodecin family protein -
LVJ71_RS08015 (LVJ71_08015) 1699985..1700383 + 399 WP_003900351.1 hypothetical protein -
LVJ71_RS08020 (LVJ71_08020) 1700428..1701456 + 1029 WP_003407612.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T228929 WP_003407593.1 NZ_CP089776:1696148-1696465 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T228929 NZ_CP124355:c4331957-4331739 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT228929 WP_003900349.1 NZ_CP089776:1695849-1696151 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT228929 NZ_CP124355:c4332357-4331983 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References