Detailed information of TA system TA228927

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PhRel-ATphRel/-
• Location: 1549087..1550137
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: phRel
• Uniprot ID: -
• Locus tag: LVJ71_RS07340
• Protein ID: WP_003912801.1
• Coordinates: 1549087..1549908 (+)
• Length: 274 a.a.

Antitoxin (Protein)

• Gene name: ATphRel
• Uniprot ID: A0A7U4BTV8
• Locus tag: LVJ71_RS07345
• Protein ID: WP_003407167.1
• Coordinates: 1549901..1550137 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS07320 (LVJ71_07320)	1544645..1545307	-	663	WP_003407152.1	membrane protein	-
LVJ71_RS07325 (LVJ71_07325)	1545304..1546089	-	786	WP_003407157.1	membrane protein	-
LVJ71_RS07330 (LVJ71_07330)	1546380..1548341	-	1962	WP_003407159.1	sigma-F factor regulator	-
LVJ71_RS07335 (LVJ71_07335)	1548480..1548866	-	387	WP_003898845.1	anti-sigma-F factor antagonist RsfA	-
LVJ71_RS07340 (LVJ71_07340)	1549087..1549908	+	822	WP_003912801.1	GTP pyrophosphokinase family protein	Toxin
LVJ71_RS07345 (LVJ71_07345)	1549901..1550137	+	237	WP_003407167.1	hypothetical protein	Antitoxin
LVJ71_RS07350 (LVJ71_07350)	1550209..1551414	-	1206	WP_003898846.1	serine hydrolase domain-containing protein	-
LVJ71_RS07355 (LVJ71_07355)	1551717..1552502	+	786	WP_003407180.1	lipoprotein LprF	-
LVJ71_RS07360 (LVJ71_07360)	1552698..1554167	+	1470	WP_003900326.1	cytochrome b5 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (76-174)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 274 a.a.
• Molecular weight: 30134.12 Da
• Isoelectric Point: 5.8187

>T228927 WP_003912801.1 NZ_CP089776:1549087-1549908 [Mycobacterium tuberculosis]
VVVALVGSAIVDLHSRPPWSNNAVRRLGVALRDGVDPPVDCPSYAEVMLWHADLAAEVQDRIEGRSWSASELLVTSRAKS
QDTLLAKLRRRPYLQLNTIQDIAGVRIDADFLLGEQTRLAREIADHFGADQPAIHDLRDHPHAGYRAVHVWLRLPAGRVE
IQIRTILQSLWANFYELLADAYGRGIRYDERPEQLAAGVVPAQLQELVGVMQDASADLAMHEAEWQHCAEIEYPGQRAMA
LGEASKNKATVLATTKFRLERAINEAESAGGGG

Nucleotide

• Length: 822 bp

>T228927 NZ_CP124355:3880396-3880680 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8830.90 Da
• Isoelectric Point: 7.3178

>AT228927 WP_003407167.1 NZ_CP089776:1549901-1550137 [Mycobacterium tuberculosis]
VGEVAGYVVEYNRRTHVRRITEFATPQEAMEHRLKLEAERTDSNIEIVALVSKSLGTLKQTHSRYFTGEELNVGNGAR

Nucleotide

• Length: 237 bp

>AT228927 NZ_CP124355:3880160-3880393 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BTV8