Detailed information of TA system TA228925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1391943..1392631
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ71_RS06655
• Protein ID: WP_003406304.1
• Coordinates: 1392200..1392631 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ71_RS06650
• Protein ID: WP_003406302.1
• Coordinates: 1391943..1392203 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS06630 (LVJ71_06620)	1387520..1388344	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ71_RS06635 (LVJ71_06625)	1388349..1389530	+	1182	WP_003406299.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ71_RS06640 (LVJ71_06630)	1389607..1390707	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ71_RS06645 (LVJ71_06635)	1390878..1391867	+	990	WP_003406301.1	malate dehydrogenase	-
LVJ71_RS06650 (LVJ71_06640)	1391943..1392203	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ71_RS06655 (LVJ71_06645)	1392200..1392631	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ71_RS06660 (LVJ71_06650)	1392654..1394342	-	1689	WP_003910308.1	PE family protein	-
LVJ71_RS06665 (LVJ71_06655)	1394522..1395382	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ71_RS06670 (LVJ71_06660)	1395463..1396293	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ71_RS06675 (LVJ71_06665)	1396350..1396643	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ71_RS06680 (LVJ71_06670)	1396640..1396909	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T228925 WP_003406304.1 NZ_CP089776:1392200-1392631 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T228925 NZ_CP124355:3686926-3687303 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT228925 WP_003406302.1 NZ_CP089776:1391943-1392203 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT228925 NZ_CP124355:3686468-3686836 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6