Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1030801..1031774 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name tacT Uniprot ID O05911
Locus tag LVJ71_RS04900 Protein ID WP_003404756.1
Coordinates 1031274..1031774 (+) Length 167 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID O05910
Locus tag LVJ71_RS04895 Protein ID WP_003898647.1
Coordinates 1030801..1031277 (+) Length 159 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS04875 (LVJ71_04870) 1026648..1027919 - 1272 WP_003404747.1 PPE family protein -
LVJ71_RS04880 (LVJ71_04875) 1027934..1028233 - 300 WP_003404748.1 PE family protein -
LVJ71_RS04885 (LVJ71_04880) 1028677..1030458 + 1782 WP_003404749.1 BCCT family transporter -
LVJ71_RS04890 (LVJ71_04885) 1030458..1030712 + 255 WP_003898646.1 hypothetical protein -
LVJ71_RS04895 (LVJ71_04890) 1030801..1031277 + 477 WP_003898647.1 DUF1778 domain-containing protein Antitoxin
LVJ71_RS04900 (LVJ71_04895) 1031274..1031774 + 501 WP_003404756.1 GNAT family N-acetyltransferase Toxin
LVJ71_RS04910 (LVJ71_04905) 1032087..1033406 - 1320 WP_003404757.1 IS256-like element IS1554 family transposase -
LVJ71_RS04915 (LVJ71_04910) 1033694..1034275 + 582 WP_003404760.1 IS607-like element IS1535 family transposase -
LVJ71_RS04920 (LVJ71_04915) 1034275..1035927 + 1653 WP_003404761.1 IS607 family element RNA-guided endonuclease TnpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(38-143)

Antitoxin

Vibrio phage ICP1, Orf50

(74-150)


Sequences

Toxin        


Download         Length: 167 a.a.        Molecular weight: 18048.55 Da        Isoelectric Point: 7.8659

>T228921 WP_003404756.1 NZ_CP089776:1031274-1031774 [Mycobacterium tuberculosis]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Download         Length: 501 bp

>T228921 NZ_CP124355:3054391-3054702 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 159 a.a.        Molecular weight: 17387.98 Da        Isoelectric Point: 11.2467

>AT228921 WP_003898647.1 NZ_CP089776:1030801-1031277 [Mycobacterium tuberculosis]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Download         Length: 477 bp

>AT228921 NZ_CP124355:3054703-3054993 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSZ2

Antitoxin

Source ID Structure
AlphaFold DB A5U0V8

References