Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 761160..761700 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag LVJ71_RS03510 Protein ID WP_003403376.1
Coordinates 761160..761468 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag LVJ71_RS03515 Protein ID WP_003403381.1
Coordinates 761455..761700 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS03485 (LVJ71_03480) 756475..757980 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
LVJ71_RS03490 (LVJ71_03485) 758061..759071 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
LVJ71_RS03495 (LVJ71_03490) 759459..759842 - 384 WP_003912919.1 ribonuclease VapC6 -
LVJ71_RS03500 (LVJ71_03495) 759937..760092 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ71_RS03505 (LVJ71_03500) 760168..760884 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
LVJ71_RS03510 (LVJ71_03505) 761160..761468 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LVJ71_RS03515 (LVJ71_03510) 761455..761700 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
LVJ71_RS03520 (LVJ71_03515) 761810..762247 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
LVJ71_RS03525 (LVJ71_03520) 762244..762498 - 255 WP_003911263.1 antitoxin VapB7 -
LVJ71_RS03530 (LVJ71_03525) 762612..764975 + 2364 WP_003898547.1 arylsulfatase AtsD -
LVJ71_RS03535 (LVJ71_03530) 765038..765364 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
LVJ71_RS03540 (LVJ71_03535) 765276..765614 + 339 WP_003403405.1 PIN domain-containing protein -
LVJ71_RS03545 (LVJ71_03540) 765611..765784 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T228916 WP_003403376.1 NZ_CP089776:c761468-761160 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T228916 NZ_CP124355:c2201177-2200863 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT228916 WP_003403381.1 NZ_CP089776:c761700-761455 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT228916 NZ_CP124355:c2200866-2200450 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References