Detailed information of TA system TA228915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 759459..760092
• Replicon: chromosome
• Accession: NZ_CP089776
• Organism: Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LVJ71_RS03495
• Protein ID: WP_003912919.1
• Coordinates: 759459..759842 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: LVJ71_RS03500
• Protein ID: WP_003403368.1
• Coordinates: 759937..760092 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ71_RS03470 (LVJ71_03465)	754751..755287	+	537	WP_003403341.1	50S ribosomal protein L10	-
LVJ71_RS03475 (LVJ71_03470)	755324..755716	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
LVJ71_RS03480 (LVJ71_03475)	755709..756404	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
LVJ71_RS03485 (LVJ71_03480)	756475..757980	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
LVJ71_RS03490 (LVJ71_03485)	758061..759071	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
LVJ71_RS03495 (LVJ71_03490)	759459..759842	-	384	WP_003912919.1	ribonuclease VapC6	Toxin
LVJ71_RS03500 (LVJ71_03495)	759937..760092	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ71_RS03505 (LVJ71_03500)	760168..760884	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
LVJ71_RS03510 (LVJ71_03505)	761160..761468	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
LVJ71_RS03515 (LVJ71_03510)	761455..761700	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
LVJ71_RS03520 (LVJ71_03515)	761810..762247	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
LVJ71_RS03525 (LVJ71_03520)	762244..762498	-	255	WP_003911263.1	antitoxin VapB7	-
LVJ71_RS03530 (LVJ71_03525)	762612..764975	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13989.76 Da
• Isoelectric Point: 6.0803

>T228915 WP_003912919.1 NZ_CP089776:c759842-759459 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTATARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T228915 NZ_CP124355:2035573-2035950 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT228915 WP_003403368.1 NZ_CP089776:c760092-759937 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT228915 NZ_CP124355:2035116-2035484 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8