Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 723967..724631 Replicon chromosome
Accession NZ_CP089776
Organism Mycobacterium tuberculosis strain 02-R1896

Toxin (Protein)


Gene name vapC Uniprot ID P96917
Locus tag LVJ71_RS03325 Protein ID WP_003403246.1
Coordinates 724224..724631 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WF18
Locus tag LVJ71_RS03320 Protein ID WP_003403244.1
Coordinates 723967..724227 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ71_RS03295 (LVJ71_03290) 720144..721208 + 1065 WP_003898532.1 zinc ribbon domain-containing protein -
LVJ71_RS03300 (LVJ71_03295) 721312..722259 + 948 WP_003403232.1 DUF732 domain-containing protein -
LVJ71_RS03305 (LVJ71_03300) 722352..722606 + 255 WP_003403235.1 type II toxin-antitoxin system antitoxin VapB30 -
LVJ71_RS03310 (LVJ71_03305) 722606..723001 + 396 WP_003403236.1 type II toxin-antitoxin system toxin ribonuclease C30 -
LVJ71_RS03315 (LVJ71_03310) 723095..723835 - 741 WP_003403239.1 TVP38/TMEM64 family protein -
LVJ71_RS03320 (LVJ71_03315) 723967..724227 + 261 WP_003403244.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ71_RS03325 (LVJ71_03320) 724224..724631 + 408 WP_003403246.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ71_RS03330 (LVJ71_03325) 724703..725854 - 1152 WP_003403248.1 FIST N-terminal domain-containing protein -
LVJ71_RS03335 (LVJ71_03330) 725947..727674 - 1728 WP_003900979.1 exodeoxyribonuclease V subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-57)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14376.37 Da        Isoelectric Point: 4.3568

>T228914 WP_003403246.1 NZ_CP089776:724224-724631 [Mycobacterium tuberculosis]
VSTTPAAGVLDTSVFIATESGRQLDEALIPDRVATTVVTLAELRVGVLAAATTDIRAQRLATLESVADMETLPVDDDAAR
MWARLRIHLAESGRRVRINDLWIAAVAASRALPVITQDDDFAALDGAASVEIIRV

Download         Length: 408 bp

>T228914 NZ_CP124355:c1890610-1890203 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9492.84 Da        Isoelectric Point: 11.9108

>AT228914 WP_003403244.1 NZ_CP089776:723967-724227 [Mycobacterium tuberculosis]
MSEVASRELRNDTAGVLRRVRAGEDVTITVSGRPVAVLTPVRPRRRRWLSKTEFLSRLRGAQADPGLRNDLAVLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT228914 NZ_CP124355:c1890857-1890591 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3DBO

Antitoxin

Source ID Structure
PDB 3DBO
AlphaFold DB A0A7U4BSE4

References