Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-Phd
Location 3179189..3179737 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name relE Uniprot ID G0TFG5
Locus tag LVJ70_RS15140 Protein ID WP_003414602.1
Coordinates 3179474..3179737 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID O33347
Locus tag LVJ70_RS15135 Protein ID WP_003414599.1
Coordinates 3179189..3179470 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS15110 (LVJ70_15110) 3174810..3175667 - 858 WP_003899513.1 type I methionyl aminopeptidase -
LVJ70_RS15115 (LVJ70_15115) 3175709..3176295 - 587 Protein_2984 DUF1707 domain-containing protein -
LVJ70_RS15120 (LVJ70_15120) 3176399..3176647 + 249 WP_003913411.1 antitoxin VapB23 -
LVJ70_RS15125 (LVJ70_15125) 3176644..3177024 + 381 WP_003414592.1 type II toxin-antitoxin system VapC family toxin -
LVJ70_RS15130 (LVJ70_15130) 3177106..3178917 - 1812 WP_003414596.1 penicillin-binding protein -
LVJ70_RS15135 (LVJ70_15135) 3179189..3179470 + 282 WP_003414599.1 type II toxin-antitoxin system antitoxin RelF Antitoxin
LVJ70_RS15140 (LVJ70_15140) 3179474..3179737 + 264 WP_003414602.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LVJ70_RS15145 (LVJ70_15145) 3180110..3180964 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
LVJ70_RS15150 (LVJ70_15150) 3181020..3182183 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
LVJ70_RS15155 (LVJ70_15155) 3182200..3183414 - 1215 WP_003899518.1 zinc metalloprotease Rip -
LVJ70_RS15160 (LVJ70_15160) 3183422..3184663 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10204.82 Da        Isoelectric Point: 11.5078

>T228895 WP_003414602.1 NZ_CP089775:3179474-3179737 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Download         Length: 264 bp

>T228895 NZ_CP124353:3054307-3054618 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10183.40 Da        Isoelectric Point: 4.3440

>AT228895 WP_003414599.1 NZ_CP089775:3179189-3179470 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Download         Length: 282 bp

>AT228895 NZ_CP124353:3054619-3054909 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3G5O

Antitoxin

Source ID Structure
PDB 3G5O
AlphaFold DB A0A7U4BWP8

References