Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3138272..3138876 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag LVJ70_RS14940 Protein ID WP_003414492.1
Coordinates 3138272..3138664 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag LVJ70_RS14945 Protein ID WP_003414495.1
Coordinates 3138661..3138876 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS14910 (LVJ70_14915) 3133422..3134210 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
LVJ70_RS14915 (LVJ70_14920) 3134544..3135089 - 546 WP_003913758.1 DUF1802 family protein -
LVJ70_RS14920 (LVJ70_14925) 3135361..3136245 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
LVJ70_RS14925 (LVJ70_14930) 3136248..3137135 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
LVJ70_RS14930 (LVJ70_14935) 3137440..3137985 - 546 WP_003899500.1 DUF1802 family protein -
LVJ70_RS14935 (LVJ70_14940) 3137982..3138251 - 270 WP_003912856.1 DUF2277 family protein -
LVJ70_RS14940 (LVJ70_14945) 3138272..3138664 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ70_RS14945 (LVJ70_14950) 3138661..3138876 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LVJ70_RS14950 (LVJ70_14955) 3138923..3139672 + 750 WP_003414497.1 enoyl-CoA hydratase -
LVJ70_RS14955 (LVJ70_14960) 3139751..3140833 - 1083 WP_003414499.1 sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC -
LVJ70_RS14960 (LVJ70_14965) 3140826..3142136 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
LVJ70_RS14965 (LVJ70_14970) 3142139..3142966 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
LVJ70_RS14970 (LVJ70_14975) 3142963..3143874 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T228894 WP_003414492.1 NZ_CP089775:c3138664-3138272 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T228894 NZ_CP124353:c2656834-2656727 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT228894 WP_003414495.1 NZ_CP089775:c3138876-3138661 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT228894 NZ_CP124353:2656883-2656948 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References