Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2870241..2870878 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag LVJ70_RS13465 Protein ID WP_003413180.1
Coordinates 2870241..2870636 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag LVJ70_RS13470 Protein ID WP_003413183.1
Coordinates 2870633..2870878 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS13425 (LVJ70_13425) 2866326..2866985 + 660 WP_003900846.1 LppA family lipoprotein -
LVJ70_RS13430 (LVJ70_13430) 2866982..2867641 + 660 WP_003900847.1 LppA family lipoprotein -
LVJ70_RS13435 (LVJ70_13435) 2867638..2868300 + 663 WP_003900848.1 LppA family lipoprotein -
LVJ70_RS13440 (LVJ70_13440) 2868297..2868575 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
LVJ70_RS13445 (LVJ70_13445) 2868668..2869081 + 414 WP_003413164.1 PIN domain nuclease -
LVJ70_RS13450 (LVJ70_13450) 2869120..2869377 + 258 WP_003413167.1 CopG family transcriptional regulator -
LVJ70_RS13455 (LVJ70_13455) 2869374..2869751 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
LVJ70_RS13460 (LVJ70_13460) 2869767..2870141 - 375 WP_003413177.1 hypothetical protein -
LVJ70_RS13465 (LVJ70_13465) 2870241..2870636 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
LVJ70_RS13470 (LVJ70_13470) 2870633..2870878 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
LVJ70_RS13475 (LVJ70_13475) 2871289..2871708 - 420 WP_003413190.1 A24 family peptidase -
LVJ70_RS13480 (LVJ70_13480) 2871720..2872529 - 810 WP_003413193.1 shikimate dehydrogenase -
LVJ70_RS13485 (LVJ70_13485) 2872526..2873779 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
LVJ70_RS13490 (LVJ70_13490) 2873772..2874284 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T228887 WP_003413180.1 NZ_CP089775:c2870636-2870241 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T228887 NZ_CP124353:c919183-918932 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT228887 WP_003413183.1 NZ_CP089775:c2870878-2870633 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT228887 NZ_CP124353:c918935-918681 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References