Detailed information of TA system TA228876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mbcAT/RES-TIGR02293
• Location: 2234656..2235554
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: mbcT
• Uniprot ID: P64908
• Locus tag: LVJ70_RS10450
• Protein ID: WP_003410001.1
• Coordinates: 2234656..2235216 (-)
• Length: 187 a.a.

Antitoxin (Protein)

• Gene name: mbcA
• Uniprot ID: P64910
• Locus tag: LVJ70_RS10455
• Protein ID: WP_003410003.1
• Coordinates: 2235213..2235554 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS10420 (LVJ70_10430)	2229825..2230478	-	654	WP_003409976.1	cutinase Cfp21	-
LVJ70_RS10425 (LVJ70_10435)	2230593..2230823	+	231	WP_003409980.1	DUF167 domain-containing protein	-
LVJ70_RS10430 (LVJ70_10440)	2230908..2231819	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
LVJ70_RS10435 (LVJ70_10445)	2231928..2232527	+	600	WP_003409989.1	L-lysine exporter	-
LVJ70_RS10440 (LVJ70_10450)	2232943..2233371	+	429	WP_003409992.1	cellulose-binding protein	-
LVJ70_RS10445 (LVJ70_10455)	2233597..2234136	+	540	WP_003900446.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37)	-
LVJ70_RS10450 (LVJ70_10460)	2234656..2235216	-	561	WP_003410001.1	RES family NAD+ phosphorylase	Toxin
LVJ70_RS10455 (LVJ70_10465)	2235213..2235554	-	342	WP_003410003.1	DUF2384 domain-containing protein	Antitoxin
LVJ70_RS10460 (LVJ70_10470)	2235640..2235897	+	258	WP_003410006.1	hypothetical protein	-
LVJ70_RS10465 (LVJ70_10475)	2235798..2236133	-	336	WP_003410009.1	dehydrogenase	-
LVJ70_RS10470 (LVJ70_10480)	2236222..2236566	-	345	WP_003410010.1	type II toxin-antitoxin system toxin endoribonuclease MazF6	-
LVJ70_RS10475 (LVJ70_10485)	2236560..2236808	-	249	WP_003410014.1	ribbon-helix-helix protein, CopG family	-
LVJ70_RS10480 (LVJ70_10490)	2236908..2239223	-	2316	WP_003899120.1	cation transporter ATPase CptG	-
LVJ70_RS10485 (LVJ70_10495)	2239220..2239492	-	273	WP_003410017.1	DUF1490 family protein	-
LVJ70_RS10490 (LVJ70_10500)	2239545..2239901	-	357	WP_003410018.1	Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (25-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (69-110)

Sequences

Toxin

Protein

• Length: 187 a.a.
• Molecular weight: 20248.70 Da
• Isoelectric Point: 4.5091

>T228876 WP_003410001.1 NZ_CP089775:c2235216-2234656 [Mycobacterium tuberculosis]
VSDALDEGLVQRIDARGTIEWSETCYRYTGAHRDALSGEGARRFGGRWNPPLLFPAIYLADSAQACMVEVERAAQAASTT
AEKMLEAAYRLHTIDVTDLAVLDLTTPQAREAVGLENDDIYGDDWSGCQAVGHAAWFLHMQGVLVPAAGGVGLVVTAYEQ
RTRPGQLQLRQSVDLTPALYQELRAT

Nucleotide

• Length: 561 bp

>T228876 NZ_CP124351:4147802-4148722 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12488.33 Da
• Isoelectric Point: 4.8359

>AT228876 WP_003410003.1 NZ_CP089775:c2235554-2235213 [Mycobacterium tuberculosis]
MGVNVLASTVSGAIERLGLTYEEVGDIVDASPRSVARWTAGQVVPQRLNKQRLIELAYVADALAEVLPRDQANVWMFSPN
RLLEHRKPADLVRDGEYQRVLALIDAMAEGVFV

Nucleotide

• Length: 342 bp

>AT228876 NZ_CP124351:4147033-4147815 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• PDB: 6FKG

Antitoxin

• AlphaFold DB: A0A7U4FAE4