Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2227330..2228018 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name vapC Uniprot ID P9WF64
Locus tag LVJ70_RS10405 Protein ID WP_003899117.1
Coordinates 2227330..2227749 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag LVJ70_RS10410 Protein ID WP_003409968.1
Coordinates 2227758..2228018 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS10385 (LVJ70_10395) 2222825..2223673 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
LVJ70_RS10390 (LVJ70_10400) 2223636..2225081 - 1446 WP_003899115.1 APC family permease -
LVJ70_RS10395 (LVJ70_10405) 2225260..2225946 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
LVJ70_RS10400 (LVJ70_10410) 2226137..2227105 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
LVJ70_RS10405 (LVJ70_10415) 2227330..2227749 - 420 WP_003899117.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ70_RS10410 (LVJ70_10420) 2227758..2228018 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ70_RS10415 (LVJ70_10425) 2228161..2229837 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
LVJ70_RS10420 (LVJ70_10430) 2229825..2230478 - 654 WP_003409976.1 cutinase Cfp21 -
LVJ70_RS10425 (LVJ70_10435) 2230593..2230823 + 231 WP_003409980.1 DUF167 domain-containing protein -
LVJ70_RS10430 (LVJ70_10440) 2230908..2231819 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
LVJ70_RS10435 (LVJ70_10445) 2231928..2232527 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14748.87 Da        Isoelectric Point: 7.9722

>T228875 WP_003899117.1 NZ_CP089775:c2227749-2227330 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDHEFRGVVGYWPGVA

Download         Length: 420 bp

>T228875 NZ_CP124351:3880068-3880352 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT228875 WP_003409968.1 NZ_CP089775:c2228018-2227758 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT228875 NZ_CP124351:3879832-3880065 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7TLI0

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References