Detailed information of TA system TA228872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2196560..2197263
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: LVJ70_RS10195
• Protein ID: WP_003409778.1
• Coordinates: 2196560..2196889 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: LVJ70_RS10200
• Protein ID: WP_003409780.1
• Coordinates: 2196886..2197263 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS10175 (LVJ70_10185)	2192943..2194013	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
LVJ70_RS10180 (LVJ70_10190)	2194010..2194525	+	516	WP_003409718.1	flavin reductase family protein	-
LVJ70_RS10185 (LVJ70_10195)	2194522..2195583	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
LVJ70_RS10190 (LVJ70_10200)	2195580..2196350	+	771	WP_003409775.1	SDR family oxidoreductase	-
LVJ70_RS10195 (LVJ70_10205)	2196560..2196889	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVJ70_RS10200 (LVJ70_10210)	2196886..2197263	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
LVJ70_RS10205 (LVJ70_10215)	2197260..2197850	-	591	WP_003409784.1	SEC-C metal-binding domain-containing protein	-
LVJ70_RS10210 (LVJ70_10220)	2197905..2199269	+	1365	WP_003903691.1	HNH endonuclease signature motif containing protein	-
LVJ70_RS10215 (LVJ70_10225)	2199424..2199876	-	453	WP_003899095.1	lipoprotein	-
LVJ70_RS10220 (LVJ70_10230)	2199940..2200341	+	402	WP_003409869.1	hypothetical protein	-
LVJ70_RS10225 (LVJ70_10235)	2200334..2200516	-	183	WP_003409870.1	hypothetical protein	-
LVJ70_RS10230 (LVJ70_10240)	2200630..2200980	-	351	WP_003899096.1	hypothetical protein	-
LVJ70_RS10235 (LVJ70_10245)	2200991..2201893	-	903	WP_003899097.1	hypothetical protein	-
LVJ70_RS10240 (LVJ70_10250)	2201914..2202105	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T228872 WP_003409778.1 NZ_CP089775:c2196889-2196560 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T228872 NZ_CP124351:3589808-3590158 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT228872 WP_003409780.1 NZ_CP089775:c2197263-2196886 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT228872 NZ_CP124351:3589563-3589814 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8