Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1942133..1942746 Replicon chromosome
Accession NZ_CP089775
Organism Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag LVJ70_RS09075 Protein ID WP_003408465.1
Coordinates 1942133..1942522 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag LVJ70_RS09080 Protein ID WP_003408469.1
Coordinates 1942519..1942746 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ70_RS09040 (LVJ70_09045) 1937762..1938676 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
LVJ70_RS09045 (LVJ70_09050) 1938679..1939509 + 831 WP_003408448.1 cyclase family protein -
LVJ70_RS09050 (LVJ70_09055) 1939509..1939859 + 351 WP_003898986.1 cupin domain-containing protein -
LVJ70_RS09055 (LVJ70_09060) 1939912..1940730 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
LVJ70_RS09060 (LVJ70_09065) 1940744..1941523 + 780 WP_003408460.1 IclR family transcriptional regulator -
LVJ70_RS09070 (LVJ70_09075) 1941954..1942085 + 132 Protein_1789 IS3 family transposase -
LVJ70_RS09075 (LVJ70_09080) 1942133..1942522 - 390 WP_003408465.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ70_RS09080 (LVJ70_09085) 1942519..1942746 - 228 WP_003408469.1 antitoxin Antitoxin
LVJ70_RS09085 (LVJ70_09090) 1942964..1944448 + 1485 WP_003408473.1 biotin carboxylase -
LVJ70_RS09090 (LVJ70_09095) 1944445..1945692 + 1248 WP_003408476.1 serine hydrolase -
LVJ70_RS09095 (LVJ70_09100) 1945735..1946154 - 420 WP_003408483.1 hypothetical protein -
LVJ70_RS09100 (LVJ70_09105) 1946144..1946854 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T228870 WP_003408465.1 NZ_CP089775:c1942522-1942133 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T228870 NZ_CP124351:3370627-3370800 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT228870 WP_003408469.1 NZ_CP089775:c1942746-1942519 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT228870 NZ_CP124351:3370303-3370599 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References