Detailed information of TA system TA022887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 446947..447529
• Replicon: chromosome
• Accession: NC_010725
• Organism: Methylobacterium populi BJ001

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: B1ZJ21
• Locus tag: MPOP_RS02070
• Protein ID: WP_012452354.1
• Coordinates: 447254..447529 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: B1ZJ20
• Locus tag: MPOP_RS02065
• Protein ID: WP_012452353.1
• Coordinates: 446947..447264 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPOP_RS02050	442303..443277	-	975	WP_012452350.1	aliphatic sulfonate ABC transporter substrate-binding protein	-
MPOP_RS02055	443437..446223	+	2787	WP_012452351.1	[protein-PII] uridylyltransferase	-
MPOP_RS02060	446316..446924	+	609	WP_012452352.1	nucleotide exchange factor GrpE	-
MPOP_RS02065	446947..447264	-	318	WP_012452353.1	BrnA antitoxin family protein	Antitoxin
MPOP_RS02070	447254..447529	-	276	WP_012452354.1	BrnT family toxin	Toxin
MPOP_RS02075	447624..448481	-	858	WP_041371839.1	prephenate dehydratase	-
MPOP_RS02080	448497..449240	-	744	WP_012452356.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
MPOP_RS27355	449570..450493	+	924	WP_012452357.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-84)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-100)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10130.63 Da
• Isoelectric Point: 8.7077

>T22887 WP_012452354.1 NC_010725:c447529-447254 [Methylorubrum populi BJ001]
MLIVWDEPKRLTNLQKHGLDFADFEAGFDFETALVEGARSSALGSARMKVIGELDGRIVVAAIITPLGQEAISLISLRRA
SRSERRRYDAR

Nucleotide

• Length: 276 bp

>T22887 NC_010725:c447529-447254 [Methylorubrum populi BJ001]
ATGTTGATCGTCTGGGACGAGCCCAAGCGCCTGACCAATCTGCAGAAGCACGGCCTCGACTTTGCGGATTTCGAAGCAGG
CTTCGACTTCGAGACCGCTCTCGTCGAGGGAGCGCGATCGAGTGCACTCGGTTCGGCCCGGATGAAGGTCATCGGCGAAC
TCGACGGACGGATCGTGGTGGCGGCAATCATCACGCCACTGGGACAGGAGGCGATATCCCTCATCAGCCTCCGCCGAGCC
AGCCGGAGCGAACGGAGGCGCTACGATGCCCGTTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11844.32 Da
• Isoelectric Point: 5.2482

>AT22887 WP_012452353.1 NC_010725:c447264-446947 [Methylorubrum populi BJ001]
MPVRKRDRSPITDAEEAEIQAGIAADPDNPEWTEEDFRSAVPFAEAFPTLTEKLRRTRGAQKTPTKQLVSLRLDQDVLLR
FRATGPGWQARMNEALRRAAADLPD

Nucleotide

• Length: 318 bp

>AT22887 NC_010725:c447264-446947 [Methylorubrum populi BJ001]
ATGCCCGTTAGAAAGCGCGATCGTTCACCGATCACCGATGCCGAGGAGGCGGAGATCCAGGCCGGCATCGCGGCCGATCC
CGACAACCCGGAATGGACCGAGGAAGACTTTCGAAGCGCCGTGCCCTTCGCGGAAGCCTTCCCCACCCTCACCGAAAAAT
TGCGCCGTACCCGCGGCGCGCAAAAGACTCCGACCAAGCAGCTCGTCAGCTTGAGGCTTGATCAGGACGTCCTGCTACGC
TTCAGGGCGACCGGCCCCGGCTGGCAGGCTCGGATGAACGAGGCGCTGCGCCGAGCCGCCGCGGACCTGCCCGACTGA

Structures

Toxin

• AlphaFold DB: B1ZJ21

Antitoxin

• AlphaFold DB: B1ZJ20