Detailed information of TA system TA228864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1391073..1391761
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0THR7
• Locus tag: LVJ70_RS06665
• Protein ID: WP_003406304.1
• Coordinates: 1391330..1391761 (+)
• Length: 144 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0THR6
• Locus tag: LVJ70_RS06660
• Protein ID: WP_003406302.1
• Coordinates: 1391073..1391333 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS06640 (LVJ70_06630)	1386650..1387474	+	825	WP_003900298.1	trehalose ABC transporter permease SugB	-
LVJ70_RS06645 (LVJ70_06635)	1387479..1388660	+	1182	WP_070900236.1	trehalose ABC transporter ATP-binding protein SugC	-
LVJ70_RS06650 (LVJ70_06640)	1388737..1389837	-	1101	WP_003406300.1	magnesium/cobalt transporter CorA	-
LVJ70_RS06655 (LVJ70_06645)	1390008..1390997	+	990	WP_003406301.1	malate dehydrogenase	-
LVJ70_RS06660 (LVJ70_06650)	1391073..1391333	+	261	WP_003406302.1	type II toxin-antitoxin system antitoxin VapB33	Antitoxin
LVJ70_RS06665 (LVJ70_06655)	1391330..1391761	+	432	WP_003406304.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ70_RS06670 (LVJ70_06660)	1391784..1393472	-	1689	WP_003910308.1	PE family protein	-
LVJ70_RS06675 (LVJ70_06665)	1393652..1394512	+	861	WP_003900301.1	glycine betaine ABC transporter substrate-binding protein	-
LVJ70_RS06680 (LVJ70_06670)	1394593..1395423	-	831	WP_003898788.1	SDR family NAD(P)-dependent oxidoreductase	-
LVJ70_RS06685 (LVJ70_06675)	1395480..1395773	-	294	WP_003898789.1	type II toxin-antitoxin system mRNA interferase RelE	-
LVJ70_RS06690 (LVJ70_06680)	1395770..1396039	-	270	WP_003406322.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-142)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 144 a.a.
• Molecular weight: 15873.08 Da
• Isoelectric Point: 5.8838

>T228864 WP_003406304.1 NZ_CP089775:1391330-1391761 [Mycobacterium tuberculosis]
VIIPDINLLLYAVITGFPQHRRAHAWWQDTVNGHTRIGLTYPALFGFLRIATSARVLAAPLPTADAIAYVREWLSQPNVD
LLTAGPRHLDIALGLLDKLGTASHLTTDVQLAAYGIEYDAEIHSSDTDFARFADLKWTDPLRE

Nucleotide

• Length: 432 bp

>T228864 NZ_CP124351:c2201092-2200778 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9918.25 Da
• Isoelectric Point: 6.4755

>AT228864 WP_003406302.1 NZ_CP089775:1391073-1391333 [Mycobacterium tuberculosis]
MRTTLTLDDDVVRLVEDAVHRERRPMKQVINDALRRALAPPVKRQEQYRLEPHESAVRSGLDLAGFNKLADELEDEALLD
ATRRAR

Nucleotide

• Length: 261 bp

>AT228864 NZ_CP124351:c2200781-2200365 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: G0THR7

Antitoxin

• AlphaFold DB: G0THR6