Detailed information of TA system TA228863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1246276..1246844
• Replicon: chromosome
• Accession: NZ_CP089775
• Organism: Mycobacterium tuberculosis strain 02-R1708

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: LVJ70_RS05990
• Protein ID: WP_003405865.1
• Coordinates: 1246470..1246844 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: LVJ70_RS05985
• Protein ID: WP_003405863.1
• Coordinates: 1246276..1246473 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ70_RS05965 (LVJ70_05960)	1242317..1242955	-	639	WP_003898730.1	lipid droplet-associated protein	-
LVJ70_RS05970 (LVJ70_05965)	1243045..1244052	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
LVJ70_RS05975 (LVJ70_05970)	1244069..1245052	-	984	WP_003898731.1	hypothetical protein	-
LVJ70_RS05980 (LVJ70_05975)	1245115..1246188	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
LVJ70_RS05985 (LVJ70_05980)	1246276..1246473	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LVJ70_RS05990 (LVJ70_05985)	1246470..1246844	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
LVJ70_RS05995 (LVJ70_05990)	1247047..1247745	+	699	WP_003898733.1	hypothetical protein	-
LVJ70_RS06000 (LVJ70_05995)	1247863..1248048	+	186	WP_003901093.1	hypothetical protein	-
LVJ70_RS06005 (LVJ70_06000)	1247975..1248250	-	276	WP_003405867.1	hypothetical protein	-
LVJ70_RS06010 (LVJ70_06005)	1248493..1248816	+	324	WP_003405871.1	putative quinol monooxygenase	-
LVJ70_RS06015 (LVJ70_06010)	1248831..1249529	-	699	WP_031646953.1	hypothetical protein	-
LVJ70_RS06020 (LVJ70_06015)	1249563..1249772	+	210	WP_003911400.1	hypothetical protein	-
LVJ70_RS06025 (LVJ70_06020)	1249724..1250494	-	771	Protein_1187	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T228863 WP_003405865.1 NZ_CP089775:1246470-1246844 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T228863 NZ_CP124351:2035488-2035865 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCAGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT228863 WP_003405863.1 NZ_CP089775:1246276-1246473 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT228863 NZ_CP124351:2035031-2035399 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCTCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGTACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACCGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07